Survival of bundleless hair cells and subsequent bundle replacement in the bullfrog's saccule
Survival of bundleless hair cells and subsequent bundle replacement in the bullfrog's saccule
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DOI：
10.1002/neu.10002
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发表时间：
2002-02-05
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影响因子：
--
通讯作者：
Corwin, JT
中科院分区：
文献类型：
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作者：
Gale, JE;Meyers, JR;Corwin, JT
我们的听觉和平衡感依赖于毛细胞，即内耳的感觉感受器。由于暴露于噪音、氨基糖苷类抗生素和抗肿瘤药物，导致毛细胞受损，数百万人患有听力和平衡缺陷。在某些物种中，这种损伤可以通过产生新细胞来逆转。这种增殖反应在哺乳动物中是有限的，但据推测，受损的毛细胞可能存活并进行细胞内修复。我们研究了牛蛙囊状毛细胞暴露于低剂量的氨基糖苷类抗生素庆大霉素后的命运，以确定毛细胞是否能在这种治疗中存活并随后修复。在牛蛙球囊的器官培养中，延时视频显微镜、双光子显微镜、电子显微镜和免疫细胞化学的组合表明，毛细胞可以失去它们的毛束并作为无毛细胞存活至少一周。延时和电子显微镜显示的阶段，在分离的束从细胞体。抗生素处理后2、4和7天固定的培养物的扫描电子显微镜（SEM）显示，在培养的4和7天之间产生了许多新的毛束。进一步的检查发现毛细胞与小的修复的毛束旁边的较大的幸存束受损的残余物。结果表明，感觉毛细胞可以在没有有丝分裂的情况下进行细胞内自我修复，为功能性毛细胞恢复和非增殖性恢复提供了新的可能性。(C)2002年John Wiley Sons，Inc.
our senses of hearing and balance depend upon hair cells, the sensory receptors of the inner ear. Millions of people suffer from hearing and balance deficits caused by damage to hair cells as a result of exposure to noise, aminoglycoside antibiotics, and antitumor drugs. In some species such damage can be reversed through the production of new cells. This proliferative response is limited in mammals but it has been hypothesized that damaged hair cells might survive and undergo intracellular repair. We examined the fate of bullfrog saccular hair cells after exposure to a low dose of the aminoglycoside antibiotic gentamicin to determine whether hair cells could survive such treatment and subsequently be repaired. In organ cultures of the bullfrog saccule a combination of time-lapse video microscopy, two-photon microscopy, electron microscopy, and immunocytochemistry showed that hair cells can lose their hair bundle and survive as bundleless cells for at least I week. Time-lapse and electron microscopy revealed stages in the separation of the bundle from the cell body. Scanning electron microscopy (SEM) of cultures fixed 2, 4, and 7 days after antibiotic treatment showed that numerous new hair bundles were produced between 4 and 7 days of culture. Further examination revealed hair cells with small repaired hair bundles alongside damaged remnants of larger surviving bundles. The results indicate that sensory hair cells can undergo intracellular self-repair in the absence of mitosis, offering new possibilities for functional hair cell recovery and an explanation for non-proliferative recovery. (C) 2002 John Wiley Sons, Inc.