Ion channels that control fertility in mammalian spermatozoa
Ion channels that control fertility in mammalian spermatozoa
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DOI：
10.1387/ijdb.072554bn
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发表时间：
2008-01-01
影响因子：
0.7
通讯作者：
Clapham, David E.
中科院分区：
文献类型：
--
作者：
Navarro, Betsy;Kirichok, Yuriy;Clapham, David E.
哺乳动物精子的全细胞电压钳首次实现于2006年。这一技术进步，结合遗传删除策略，使明确的识别精子离子通道电流成为可能。本文综述了哺乳动物精子中直接测量的离子通道电流及其在受精过程中的生理作用。主要电流是需要表达4个mCatSper基因的Ca2+选择性电流，以及具有与mSIo3最相似性质的整流K+电流。细胞内碱化激活这两个通道，并诱导过度激活的运动。
Whole-cell voltage clamp of mammalian spermatozoa was first achieved in 2006. This technical advance, combined with genetic deletion strategies, makes unambiguous identification of sperm ion channel currents possible. This review summarizes the ion channel currents that have been directly measured in mammalian sperm, and their physiological roles in fertilization. The predominant currents are a Ca2+-selective current requiring expression of the 4 mCatSper genes, and a rectifying K+ current with properties most similar to mSIo3. Intracellular alkalinization activates both channels and induces hyperactivated motility.