Community structure of cultivable protists in different grassland and forest soils of Thuringia
Community structure of cultivable protists in different grassland and forest soils of Thuringia
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DOI：
10.1016/j.pedobi.2012.07.001
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发表时间：
2013-01-01
期刊：
影响因子：
2.3
通讯作者：
Arndt, Hartmut
中科院分区：
文献类型：
--
作者：
Domonell, Anne;Brabender, Marian;Arndt, Hartmut
土壤微生物群研究是生物多样性研究的前沿领域之一。原生动物等食菌动物在土壤矿化过程和关键生态系统功能中起着重要作用。原生动物以根瘤菌为食，刺激微生物矿化过程，从而间接促进养分循环。了解原生动物的多样性及其基本功能对了解营养循环至关重要。然而，关于土壤中原生动物数量和分类组成的可靠数据很少。我们比较了德国8个不同草地和森林样地的小型可养殖原生动物的形态类型。调查样地在景观类型和土地利用强度上存在差异。此外，我们还改进了一种测定土壤中原生动物丰度和群落结构的液相色谱法。原生动物的丰度在1.7 ~ 12.7 × 10(4) ind.g（-1）干重之间。优势类群为各种变形虫（49.9 +/- 12.4%）和尾虫（32.4 +/- 13.2%），其次为叠层虫（7.7 +/- 4.2%）、裸藻（7.5 +/- 7.6%）、单胞虫（1.5 +/- 1.5%）和纤毛虫（0.5 +/- 0.7%）。异养鞭毛虫和裸变形虫对所有地点的总原生动物丰度的贡献相似。与森林样地相比，草地样地的原生动物丰度略高，多样性更大。两种生境类型在粗略的分类水平上表现出惊人的相似，但在更高的分类分辨率上表现出更多样化的模式。(C) 2012 Elsevier GmbH版权所有。
Research on soil microbiota is one of the frontiers in biodiversity research. Bacterivores, such as protozoans, contribute significantly to mineralization processes and key ecosystem functions in soil. Protozoan grazing on rhizobacteria stimulates microbial mineralization processes and thereby indirectly nutrient cycling. Understanding the diversity of protozoans and their fundamental functions is essential for knowledge of nutrient cycling. However, reliable data on protozoan numbers and taxonomic composition in soil are scarce. We compared the occurrence of morphotypes of small cultivable protozoans of eight different grassland and forest sites in Germany. The investigated sites differed both in landscape type and intensity of land use. Additionally, we modified a liquid aliquot method to determine protozoan abundances and community structure on the morphotype level in soil. Protozoan abundances at the investigated sites ranged between 1.7 and 12.7 x 10(4) ind.g(-1) dry weight. The dominant groups were various amoebae (49.9 +/- 12.4%) and cercozoans (32.4 +/- 13.2%), followed by stramenopiles (7.7 +/- 4.2%), euglenozoans (7.5 +/- 7.6%), apusomonads (1.5 +/- 1.5%) and ciliates (0.5 +/- 0.7%).Heterotrophic flagellates and naked amoebae showed similar contributions to total protozoan abundances at all sites. Protozoan abundances were slightly higher and more variable in grassland sites compared to forest sites. Both habitat types showed a surprisingly similar community structure on a rough taxonomic level, but a more diverse pattern at a higher taxonomic resolution. (C) 2012 Elsevier GmbH. All rights reserved.