Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods
Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods
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DOI：
10.1021/ja057254a
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发表时间：
2006-02-15
影响因子：
15
通讯作者：
El-Sayed, MA
中科院分区：
文献类型：
--
作者：
Huang, XH;El-Sayed, IH;El-Sayed, MA
由于在表面处的强电场，贵金属纳米颗粒对电磁辐射的吸收和散射强烈增强。这些独特的性质提供了潜在的设计新的光学活性试剂，同时分子成像和光热癌症治疗。期望使用在辐射光谱的近红外（NIR）区域中具有活性的试剂，以最小化天然组织中固有发色团的光消光。具有合适纵横比（长度除以宽度）的金纳米棒可以在NIR区域（650-900 nm）中强烈吸收和散射。在目前的工作中，我们提供了一个在体外演示的金纳米棒作为新的造影剂的分子成像和光热癌症治疗。合成纳米棒并将其与抗表皮生长因子受体（抗EGFR）单克隆抗体结合，并在细胞培养物中与非恶性上皮细胞系（HaCat）和两种恶性口腔上皮细胞系（HOC 313克隆8和HSC 3）一起孵育。由于恶性细胞的细胞质膜上过表达的EGFR，抗EGFR抗体缀合的纳米棒以高得多的亲和力特异性结合恶性型细胞的表面。由于使用实验室显微镜观察到暗视野中金纳米棒的强烈散射红光，恶性细胞被清楚地可视化并从非恶性细胞中诊断出来。研究发现，在800 nm连续红光照射下，恶性细胞的光热破坏所需能量约为非恶性细胞的一半。因此，同时实现了有效的癌细胞诊断和选择性光热治疗。
Due to strong electric fields at the surface, the absorption and scattering of electromagnetic radiation by noble metal nanoparticles are strongly enhanced. These unique properties provide the potential of designing novel optically active reagents for simultaneous molecular imaging and photothermal cancer therapy. It is desirable to use agents that are active in the near-infrared (NIR) region of the radiation spectrum to minimize the light extinction by intrinsic chromophores in native tissue. Gold nanorods with suitable aspect ratios (length divided by width) can absorb and scatter strongly in the NIR region (650-900 nm). In the present work, we provide an in vitro demonstration of gold nanorods as novel contrast agents for both molecular imaging and photothermal cancer therapy. Nanorods are synthesized and conjugated to anti-epidermal growth factor receptor (anti-EGFR) monoclonal antibodies and incubated in cell cultures with a nonmalignant epithelial cell line (HaCat) and two malignant oral epithelial cell lines (HOC 313 clone 8 and HSC 3). The anti-EGFR anti body-conjugated nanorods bind specifically to the surface of the malignant-type cells with a much higher affinity due to the overexpressed EGFR on the cytoplasmic membrane of the malignant cells. As a result of the strongly scattered red light from gold nanorods in dark field, observed using a laboratory microscope, the malignant cells are clearly visualized and diagnosed from the nonmalignant cells. It is found that, after exposure to continuous red laser at 800 nm, malignant cells require about half the laser energy to be photothermally destroyed than the nonmalignant cells. Thus, both efficient cancer cell diagnostics and selective photothermal therapy are realized at the same time.