Improved HPLC determination of 5-S-cysteinyldopa in serum.
Improved HPLC determination of 5-S-cysteinyldopa in serum.
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改进了血清中 5-S-半胱氨酰多巴的 HPLC 测定。
DOI：
10.1093/clinchem/40.3.495
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发表时间：
1994
影响因子：
9.3
通讯作者：
S. Ito
中科院分区：
文献类型：
--
作者：
K. Wakamatsu;S. Ito
5- s - cd在恶性黑色素瘤中的作用，为了应对血清中5- s - cd分析需求的增加，我们改进了我们原来HPLC测定5- s - cd的方法(5)。在用HCIO4洗脱5-S-CD之前，用pH为4.0的磷酸盐缓冲液洗涤氧化铝，这不仅可以去除保留时间较长的化合物，还可以去除其他杂质，我们发现可以将每次色谱运行的时间从90 miii缩短到45 miii。进一步的改进是用5- s -半胱氨酸-a-甲基多巴（Me-CD）代替2-甲基-3-（3,4 -二羟基苯基）- dl -丙氨酸（a-甲基多巴）作为内标。
5-S-CD in malignant melanoma, and, to cope with the increasing need to analyze 5-S-CD in serum, we have im-proved our original method for HPLC determination of 5-S-CD (5). By wash-ing alumina with a phosphate buffer, pH 4.0, before the elution of 5-S-CD with HCIO4, which removes not only compounds with longer retention times but also other impurities, we found it possible to shorten the time for each chromatographic run from 90 miii to 45 miii. Further improvement was achieved by replacing, as internal standard, 2-methyl-3-(3, 4-dihydroxyphenyl)-DL-alanine(a-methyl-DOPA) with 5-S-cysteinyl-a-methyldopa(Me-CD).