Sexually dimorphic expression of estrogen receptor β in the anteroventral periventricular nucleus of the rat preoptic area:: Implication in luteinizing hormone surge
Sexually dimorphic expression of estrogen receptor β in the anteroventral periventricular nucleus of the rat preoptic area:: Implication in luteinizing hormone surge
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DOI：
10.1073/pnas.052707299
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发表时间：
2002-03-05
影响因子：
11.1
通讯作者：
Sakuma, Y
中科院分区：
文献类型：
--
作者：
Orikasa, C;Kondo, Y;Sakuma, Y
通过非同位素原位杂交和免疫组织化学检测，大鼠视前区前腹侧室周核（AVPV）雌激素受体（ER）0 mRNA和蛋白表达存在显着的性别差异。在距第三脑室室管膜内层 50 微米以内的 AVPV 最内侧部分，女性中观察到的 ERbeta mRNA 阳性细胞数量明显多于男性。 7、14、21、35 和 60 天（d 1 = 出生日）的大鼠表现出性别差异。在第 14 天检查时，雄性新生儿的睾丸切除术或雌性幼崽的雌激素治疗逆转了大脑表型。在成年雌性的 AVPV 中，ERα 免疫反应性共定位于 83% 的 ER mRNA 阳性细胞。酪氨酸羟化酶免疫反应性共定位于 21 天雌性中 18% 的 ERbeta 免疫反应性细胞。将 ERbeta 反义寡核苷酸输注到 AVPV 附近的第三脑室，导致连续发情天数显着延长，并且 AVPV 中 ERbeta 免疫反应细胞的数量减少 50%。这些发现支持了以下假设：AVPV 中 ERbeta 的激活是雌激素诱导女性典型黄体生成素激增的重要调节事件。
Striking sex difference was detected in the expression of estrogen receptor (ER) 0 mRNA and protein by nonisotopic in situ hybridization and immunohistochemistry in the anteroventral periventricular nucleus (AVPV) of the rat preoptic area. In females more than in males, a significantly larger number of ERbeta mRNA-positive cells were visualized in the medial-most portion of the AVPV within 50 mum from the ependymal lining of the third ventricle. Rats of 7, 14, 21, 35, and 60 days of age (d 1 = day of birth) showed the sex difference. Orchidectomy of male neonates or estrogen treatment of female pups reversed the brain phenotype when examined on d 14. In the AVPV of adult females, ERalpha immunoreactivity colocalized in 83% of ER mRNA-positive cells. Tyrosine hydroxylase immunoreactivity colocalized in 18% of ERbeta immunoreactive cells in d 21 females. infusion of an ERbeta antisense oligonucleotide into the third ventricle in the vicinity of the AVPV resulted in significantly longer days of successive estrus and a 50% reduction in the number of ERbeta-immunoreactive cells in the AVPV. These findings provide support for the hypothesis that activation of ERbeta in the AVPV is an important regulatory event in the female-typical induction of luteinizing hormone surge by estrogen.