Stoichiometric coupling of brain glucose metabolism and glutamatergic neuronal activity
Stoichiometric coupling of brain glucose metabolism and glutamatergic neuronal activity
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DOI：
10.1073/pnas.95.1.316
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发表时间：
1998-01-06
影响因子：
11.1
通讯作者：
Shulman, RG
中科院分区：
文献类型：
--
作者：
Sibson, NR;Dhankhar, A;Shulman, RG
为了确定大脑 Glc 代谢与谷氨酸神经元功能之间的关系，我们使用 C-13 NMR 光谱同时测量大鼠体内皮质中三羧酸循环和 Gin 合成的速率，根据这些测量，我们计算了神经元之间氧化 Glc 代谢和谷氨酸神经递质循环的速率 和星形胶质细胞（谷氨酸神经元活动的定量测量），通过测量一系列突触活动中三羧酸循环和杜松子酒合成的速率，我们确定了皮质中氧化葡萄糖代谢和谷氨酸神经递质循环之间的化学计量接近1:1。这一发现表明，大部分皮质能量产生支持功能性（突触）谷氨酸能神经元活动。该结果的另一个含义是，绘制皮质氧化葡萄糖代谢图谱的大脑激活研究提供了突触谷氨酸释放的定量测量。
To determine the relationship between cerebral Glc metabolism and glutamatergic neuronal function, we used C-13 NMR spectroscopy to measure, simultaneously, the rates of the tricarboxylic acid cycle and Gin synthesis in the rat cortex in vivo, From these measurements, we calculated the rates of oxidative Glc metabolism and glutamate-neurotransmitter cycling between neurons and astrocytes (a quantitative measure of glutamatergic neuronal activity), By measuring the rates of the tricarboxylic acid cycle and Gin synthesis over a range of synaptic activity, we have determined the stoichiometry between oxidative Glc metabolism and glutamate-neurotransmitter cycling in the cortex to be close to 1:1. This finding indicates that the majority of cortical energy production supports functional (synaptic) glutamatergic neuronal activity, Another implication of this result is that brain activation studies, which map cortical oxidative Glc metabolism, provide a quantitative measure of synaptic glutamate release.