Current status of sperm cryopreservation in biomedical research fish models: Zebrafish, medaka, and Xiphophorus
Current status of sperm cryopreservation in biomedical research fish models: Zebrafish, medaka, and Xiphophorus
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DOI：
10.1016/j.cbpc.2008.07.005
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发表时间：
2009-03-01
影响因子：
3.9
通讯作者：
Tiersch, Terrence R.
中科院分区：
文献类型：
--
作者：
Yang, Huiping;Tiersch, Terrence R.
观赏鱼变得越来越重要，因为它们在生物医学研究和观赏鱼贸易中的价值，而且因为许多观赏鱼在野外已受到威胁或濒临灭绝。本文综述了斑马鱼、青鳉和剑鱼属活体鱼类这三种广泛应用于生物医学研究的鱼类精子冷冻保存的现状，并重点关注观赏鱼冷冻保存未来研究和应用的需求和机遇。首先，我们总结了自然栖息地睾丸结构、精子发生、精子形态和精子生理学的基本生物学特征。其次，我们比较精子冷冻保存方案的开发。第三，我们强调人工受精在精子冷冻保存中评估解冻精子活力的重要性。最后，我们展望了精子冷冻保存的未来研究方向以及该技术在水族馆物种中的应用。 (C) 2008 Elsevier Inc. 保留所有权利。
Aquarium fishes are becoming increasingly important because of their value in biomedical research and the ornamental fish trade, and because many have become threatened or endangered in the wild. This review summarizes the current status of sperm cryopreservation in three fishes widely used in biomedical research: zebrafish, medaka, and live-bearing fishes of the genus Xiphophorus, and will focus on the needs and opportunities for future research and application of cryopreservation in aquarium fish. Firsts we summarize the basic biological characteristics regarding natural habitat testis structure, spermatogenesis, sperm morphology, and sperm physiology. Second, we compare protocol development of sperm cryopreservation. Third, we emphasize the importance of artificial fertilization in sperm cryopreservation to evaluate the viability of thawed sperm. We conclude with a look to future research directions for sperm cryopreservation and the application of this technique in aquarium species. (C) 2008 Elsevier Inc. All rights reserved.