A Novel Method for High-Yield Entrapment of Solutes into Small Liposomes
A Novel Method for High-Yield Entrapment of Solutes into Small Liposomes
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DOI：
10.3109/08982100009031096
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发表时间：
2000-01
影响因子：
4.4
通讯作者：
B. Zadi;G. Gregoriadis
中科院分区：
文献类型：
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作者：
B. Zadi;G. Gregoriadis
摘要报道了一种在小脂质体中高收率包封多种溶质的方法。该程序需要将预先形成的“空”（水填充的）小单层囊泡（SLFV）（直径60-80 nm）与蔗糖和用于包埋的溶质混合。使用适当的糖与脂质质量比（例如，1-5），混合物的脱水和随后的受控再水化导致形成包埋相当大比例（高达87%）的溶质的囊泡（直径90-200 nm）。结果与溶质不同的青霉素，核黄素，阿霉素，脱氧果糖serotonin，和表皮生长因子表明，包埋值取决于糖与脂质的质量比和溶质的身份，但不上的液晶转变温度的磷脂组分的SUV。
Abstract A procedure is reported for the high-yield entrapment of a wide variety of solutes in small liposomes. The procedure entails mixing of preformed “empty” (water-filled) small unilamellar vesicles (SLFV) (60-80 nm diameter) with sucrose and the solute destined for entrapment. Using appropriate sugar to lipid mass ratios (eg. 1-5), dehydration of the mixture and subsequent controlled rehydration leads to the formation of vesicles (90-200 nm diameter) entrapping considerable proportions (up to 87%) of the solute. Results with solutes as diverse as penicillin, riboflavin, doxorubicin, desoxyfructo-seroto-nin, and epidermal growth factor suggest that entrapment values depend on the sugar to lipid mass ratio and the identity of the solute but not on the liquid crystalline transition temperature of the phospholipid component of the SUV.