A protein kinase C-encoding gene, pkcA, is essential to the viability of the filamentous fungus Aspergillus nidulans
A protein kinase C-encoding gene, pkcA, is essential to the viability of the filamentous fungus Aspergillus nidulans
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DOI：
10.1271/bbb.70409
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发表时间：
2007-11-01
影响因子：
1.6
通讯作者：
Horiuchi, Hiroyuki
中科院分区：
文献类型：
--
作者：
Ichinomiya, Masayuki;Uchida, Hirotaka;Horiuchi, Hiroyuki
从丝状真菌构巢曲霉（Aspergillusnidulans）中克隆了一个蛋白激酶C（PKC）编码基因（pkcA）。尽管我们试图分离pkcA缺失突变体，但我们仅获得了具有Delta pkcA和pkcA（+）核的异核体。异核体产生的分生孢子萌发。但是，芽管频繁裂解，没有观察到菌落形成，表明pkcA基因是A. nidulans。我们构建了在alcA启动子（alcA（p））控制下表达pkcA的条件突变体（alcA（p）-pkcA突变体）。在alcA（p）抑制条件下，它们的菌落比野生型菌株的菌落小，并且它们的菌丝经常裂解。这些表型在中等或高渗透压条件下没有得到补救;后者的生长缺陷进一步恶化。在alcA（p）诱导条件下，alcA（p）-pkcA突变体也显示出对细胞壁去稳定剂的生长敏感性。这些结果表明，PKCA在维持细胞完整性中起着重要作用。
A protein kinase C (PKC)-encoding gene (pkcA) was isolated from the filamentous fungus Aspergillus nidulans. Although we attempted to isolate pkcA deletion mutants, we obtained only heterokaryons that had both Delta pkcA and pkcA(+) nuclei. Conidia produced by the heterokaryon germinated. The germ tubes, however, lysed frequently and no colony formation was observed, indicating that the pkcA gene is essential to the viability of A. nidulans. We constructed conditional mutants (alcA(p)-pkcA mutants) that expressed pkcA under the control of the alcA promoter (alcA(p)). Under alcA(p)-repressing conditions, their colonies were smaller than those of the wild-type strains and their hyphae lysed frequently. These phenotypes were not remedied under moderate- or high-osmolarity conditions; the growth defect deteriorated further under the latter. Under alcA(p)-inducing conditions, the alcA(p)-pkcA mutants also showed growth-sensitivity to cell wall destabilizing agents. These results indicate that pkcA plays an important role in the maintenance of cell integrity.