The Yersinia enterocolitica phospholipase gene yplA is part of the flagellar regulon
The Yersinia enterocolitica phospholipase gene yplA is part of the flagellar regulon
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DOI：
10.1128/jb.182.8.2314-2320.2000
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发表时间：
2000-04-01
影响因子：
3.2
通讯作者：
Miller, VL
中科院分区：
文献类型：
--
作者：
Schmiel, DH;Young, GM;Miller, VL
小肠结肠炎耶尔森菌yplA编码一种毒力所需的磷脂酶。毒力基因通常受环境信号的调节，因此，通过yplA：：lacZY转录融合检测yplA的表达，yplA的最大表达出现在pH 6.5~7.5之间，并在对数生长期中期诱导，在核苷酸序列中发现了潜在的FNR、环状AMP(CAMP)-cAMP受体蛋白(CRP)和Sigma(F)调节位点。通过曝气、添加葡萄糖和蔗糖、高温和盐的应用来降低yplA的表达分别与FNR、cAMP-CRP和sigma(F)介导的调控一致。在flhDC和fliA缺失株中，yplA的表达降低，表明yplA是鞭毛调节子的一部分。
Yersinia enterocolitica yplA encodes a phospholipase required for virulence. Virulence genes are often regulated in response to environmental signals; therefore, yplA expression was examined using a yplA::lacZY transcriptional fusion, Maximal yplA expression occurred between pH 6.5 and pH 7.5 and was induced in the mid-logarithmic growth phase, Potential Fnr, cyclic AMP (cAMP)-cAMP receptor protein (Crp), and sigma(F) regulatory sites were identified in the nucleotide sequence. Reduction of yplA expression by aeration, addition of glucose and sucrose, and application of high temperature and salt is consistent with Fnr-, cAMP-Crp-, and sigma(F)-mediated regulation, respectively. Expression of yplA was reduced in flhDC and fliA null strains, indicating that yplA is part of the flagellar regulon.