Gap junctions mediate intercellular calcium signalling in cultured articular chondrocytes
Gap junctions mediate intercellular calcium signalling in cultured articular chondrocytes
复制标题
DOI：
10.1016/s0143-4160(96)90001-9
复制
发表时间：
1996-11-01
期刊：
影响因子：
4
通讯作者：
Vittur, F
中科院分区：
文献类型：
--
作者：
DAndrea, P;Vittur, F
缝隙连接介导的细胞间通讯涉及多种细胞功能。其中，由于缝隙连接对细胞内第二信使的通透性，信号转导可以在几个细胞之间协调。原代培养的关节软骨细胞通过有节律性地增加胞内钙离子浓度而对细胞外ATP产生反应。用Fura-2负载细胞的数字成像荧光显微镜监测融合和半融合细胞层中的钙离子。在这些条件下，钙离子尖峰在细胞间传播，引起细胞间的钙波。在融合的软骨细胞培养中，通过刮载实验中荧光黄染料的细胞间转移来评估缝隙连接的功能表达。细胞间染料转移被缝隙连接抑制剂18α-甘草次酸阻断。在成像实验中，该抑制剂导致了ATP诱导的钙振荡的同步性丧失，并阻断了机械刺激单层单个细胞所诱导的细胞内钙的传播。结论：缝隙连接介导了软骨细胞间的信号转导，并可能为协调其代谢活动提供了一种机制。
Gap junction-mediated intercellular communication has been implicated in a variety of cellular functions. Among these, signal transduction can be coordinated among several cells due to gap junctional permeability to intracellular second messengers. Chondrocytes from articular cartilage in primary culture respond to extracellular ATP by rhythmically increasing their cytosolic Ca2+ concentration. Digital imaging fluorescence microscopy of Fura-2 loaded cells was used to monitor Ca2+ in confluent and semi-confluent cell layers. Under these conditions, Ca2+ spikes propagate from cell to cell giving rise to intercellular Ca2+ waves. The functional expression of gap junctions was assessed, in confluent chondrocyte cultures, by the intercellular transfer of Lucifer yellow dye in scrape-loading experiments. Intercellular dye transfer was blocked by the gap junction inhibitor 18 alpha-glycyrrhetinic acid. In imaging experiments, the inhibitor caused the loss of synchrony of ATP-induced Ca2+ oscillations, and blocked the intercellular Ca2+ propagation induced by mechanical stimulation of a single cell in a monolayer. It is concluded that gap junctions mediate intercellular signal transduction in cartilage cells and may provide a mechanism for co-ordinating their metabolic activity.