Derivation of embryonic stem cells from Brown Norway rats blastocysts
Derivation of embryonic stem cells from Brown Norway rats blastocysts
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DOI：
10.1016/s1673-8527(09)60066-7
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发表时间：
2010-07-01
影响因子：
5.9
通讯作者：
Zhou, Qi
中科院分区：
文献类型：
--
作者：
Zhao, Xiaoyang;Lv, Zhuo;Zhou, Qi
敲除布朗挪威（BN）大鼠可能是一个有用的人类疾病模型，然而，未能获得胚胎干细胞（ES）干扰模型的进一步发展。本研究报道了一例成功的BN大鼠ES细胞的体外诱导，其诱导效率与SD大鼠相当。BN大鼠ES细胞表达关键的转录因子，并在体外分化时能够形成胚体。将BN大鼠ES细胞注入SD大鼠囊胚后，产生了高贡献的嵌合大鼠，并可存活至成年。本研究成功获得多能性大鼠ES细胞，将为将来基因敲除大鼠的制备提供有益的参考。
Knockout Brown Norway (BN) rat could be a useful disease model for human disorders, however, a failure to derive embryonic stem (ES) cells disturbs the further development of the model. In this study, we reported a case of successful derivation of the BN rat ES cells with the derivation efficiency comparable to that of Sprague Dawley (SD) rats. The BN rat ES cells expressed the key transcription factors, and were able to form embryonic bodies (EBs) when being differentiated in vitro. After injecting the BN rat ES cells into the SD rat blastocysts, high-contribution chimeric rats were generated and could survive to their adulthood. Our success in generating pluripotent rat ES cells will benefit the generation of the knockout rats in the future.