ANALYSIS OF MONOCLONAL-ANTIBODIES REACTIVE WITH THE PORCINE CD4 ANTIGEN
ANALYSIS OF MONOCLONAL-ANTIBODIES REACTIVE WITH THE PORCINE CD4 ANTIGEN
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DOI：
10.1016/0165-2427(94)90141-4
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发表时间：
1994-10-01
影响因子：
1.8
通讯作者：
SAALMUELLER, A
中科院分区：
文献类型：
--
作者：
PESCOVITZ, MD;AASTED, B;SAALMUELLER, A
作为第一届国际猪CD研讨会的结果，四种单克隆抗体（mAb）（#002, 054, 118和127）与内部标准抗猪CD4 mAb（74-12-4）紧密聚集。为了进一步表征这些单抗之间的关系，流式细胞术中使用它们来抑制74-12-4与猪淋巴细胞的结合。mAb #002（74-12-4）和#054 （PT90）完全抑制，mAb #127 （10.2H2）和#118 （b38c6）部分抑制74-12-4的结合（分别为57%和77%）。此外，没有单克隆抗体与74-12-4阴性猪株结合。我们得出结论，这四种单抗结合在猪淋巴细胞上的相同或密切相关的表位。
As result of the First International Swine CD Workshop, four monoclonal antibodies (mAb) (#002, 054, 118, and 127) clustered closely to the internal standard anti-porcine CD4 mAb, 74-12-4. To further characterize the relationship between these mAb, they were used in flow cytometry to inhibit binding of 74-12-4 to porcine lymphocytes. mAb #002 (74-12-4) and #054 (PT90) completely inhibited, while mAb #127 (10.2H2) and #118 (b38c6) partially inhibited (57% and 77% respectively), the binding of 74-12-4. Furthermore, none of the mAbs bound to a 74-12-4 negative strain of pigs. We conclude that the four mAb bind to the same, or a closely related, epitope on porcine lymphocytes.