Three-Locus Identification, Genotyping, and Antifungal Susceptibilities of Medically Important Trichosporon Species from China
Three-Locus Identification, Genotyping, and Antifungal Susceptibilities of Medically Important Trichosporon Species from China
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DOI：
10.1128/jcm.00937-11
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发表时间：
2011-11-01
影响因子：
9.4
通讯作者：
Xu, Ying-Chun
中科院分区：
文献类型：
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作者：
Guo, Li-Na;Xiao, Meng;Xu, Ying-Chun
采用常规表型和分子方法对3个参考菌株和45个临床菌株进行分析，确定中国毛孢菌株的种属和基因型。分子方法的目标位点包括内转录间隔区 (ITS) 区域、26S rRNA 基因的 D1/D2 结构域和基因间间隔区 1 (IGS1) 区域。鉴定出八种毛孢子菌，其中最常见的是 Asahii 毛孢子菌。在基于序列的分子方法中，针对 D1/D2 结构域的方法正确分配了 97.9% (47/48) 的分离株（7 个物种），而针对 ITS 和 IGS1 区域的测试正确识别了所有 48 个分离株。当商业 API 20C AUX 和 Vitek 2 Compact YST 系统根据数据库中包含的物种的生化图谱查询其生化图谱时，分别正确识别了 91.9% 和 73% 的分离株，并分别错误识别了数据库未认领的物种分离株的 63.6% 和 36.4%。 T. asahii 临床分离株的主要基因型为基因型 4（51.4%），在其他国家很少发现。伏立康唑和伊曲康唑是体外针对所有测试的毛孢菌属最有效的药物，而卡泊芬净和两性霉素 B 的活性较差。
Three reference and 45 clinical isolates of Trichosporon were analyzed by conventional phenotypic and molecular methods to determine the species and genotypes of Trichosporon isolates from China. Target loci for molecular methods included the internal transcribed spacer (ITS) region, the D1/D2 domain of the 26S rRNA gene, and the intergenic spacer 1 (IGS1) region. Identification of eight Trichosporon species was achieved, of which Trichosporon asahii was the most common. Of the sequence-based molecular methods, the one targeting the D1/D2 domain assigned 97.9% (47/48) of isolates (seven species) correctly, while tests targeting both the ITS and IGS1 regions correctly identified all 48 isolates. The commercial API 20C AUX and Vitek 2 Compact YST systems correctly identified 91.9% and 73% of isolates when their biochemical profiles were queried against those of species contained in the databases, respectively, and misidentified 63.6% and 36.4% of isolates of species that were unclaimed by the databases, respectively. The predominant genotype among T. asahii clinical isolates, genotype 4 (51.4%), is rarely found in other countries. Voriconazole and itraconazole were the most active drugs in vitro against all the Trichosporon species tested, while caspofungin and amphotericin B demonstrated poor activity.