Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method
Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method
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DOI：
10.1006/meth.2001.1262
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发表时间：
2001-12-01
期刊：
影响因子：
4.8
通讯作者：
Schmittgen, TD
中科院分区：
文献类型：
--
作者：
Livak, KJ;Schmittgen, TD
分析实时定量 PCR 实验数据的两种最常用方法是绝对定量和相对定量。绝对定量通常通过将 PCR 信号与标准曲线相关联来确定输入拷贝数。相对定量将处理组中目标转录物的 PCR 信号与另一个样品（例如未处理的对照）的 PCR 信号相关联。 2(-DeltaDeltaCr) 方法是一种从实时定量 PCR 实验中分析基因表达相对变化的便捷方法。本报告的目的是介绍 2(-DeltaDeltaCr) 方法的推导、假设和应用。此外，我们还介绍了 2(-DeltaDeltaCr) 方法的两种变体的推导和应用，这可能有助于分析实时定量 PCR 数据。 (C) 2001 年爱思唯尔科学。
The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control. The 2(-DeltaDeltaCr) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the derivation, assumptions, and applications of the 2(-DeltaDeltaCr) method. In addition, we present the derivation and applications of two variations of the 2(-DeltaDeltaCr) method that may be useful in the analysis of real-time, quantitative PCR data. (C) 2001 Elsevier science.