Extended-spectrum beta-lactamase-producing Enterobacteriacae: unexpected low prevalence of carriage in elderly French residents
Extended-spectrum beta-lactamase-producing Enterobacteriacae: unexpected low prevalence of carriage in elderly French residents
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DOI：
10.1093/ageing/afr173
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发表时间：
2012-03-01
期刊：
影响因子：
6.7
通讯作者：
van der Mee-Marquet, Nathalie
中科院分区：
文献类型：
--
作者：
Bertrand, Xavier;Amara, Marlene;van der Mee-Marquet, Nathalie
研究设计研究进行了4个月的时间。每例患者仅入选一次。从所有居民中收集微生物样本，使用两种不同的方法来调查ESBLE携带情况。LTCF居民进行了筛查ESBLE携带与直肠拭子。将拭子悬浮于0.5 ml无菌水中，并将0.1 ml悬浮液在Drigalski琼脂（Bio-Rad，Marnes-la-Coquette，France）上划线。然后应用20 μg头孢噻肟盘（Bio-Rad，Marnes-la-Coquette，France），并将平板在35 ℃孵育18小时。根据法国微生物学会抗生素谱委员会的建议[12]，使用双纸片协同试验鉴定并检测头孢噻肟纸片周围生长的细菌是否产生ESBL。NH居民的尿液样本用于ESBLE携带筛查。在收集后1小时内，将10微升第一次晨尿样品铺在ChromID ESBL选择性琼脂平板（bioMérieux，Marcy l ′ Etoile，France）上，并将平板在37 ℃下孵育48小时。使用Vitek® 2革兰氏阴性鉴定卡（bioMérieux，Marcy l 'Etoile，France）鉴定形成菌落的细菌，并如上所述研究ESBL产生。
Study designThe study was carried-out over a 4-month period. Each patient was included only once. Microbiological samples were collected from all residents included; two different approaches were used to investigate ESBLE carriage. LTCF residents were screened for ESBLE carriage with rectal swabbing. Swabs were suspended in 0.5 ml of sterile water and 0.1 ml of the suspension was streaked on Drigalski agar (Bio-Rad, Marnes-la-Coquette, France). A 20 μg cefotaxim disk (Bio-Rad, Marnes-la-Coquette, France) was then applied, and the plated incubated for 18 h at 35 C. Bacteria growing around the cefotaxim disk were identified and tested for ESBL production using the double disk synergy test according to the recommendations of the Antibiogram Committee of the French Society for Microbiology [12]. Urine samples from NH residents were used for screening for ESBLE carriage. Ten microlitre samples of the first morning urine were plated within 1 h of collection on a ChromID ESBL selective agar plate (bioMérieux, Marcy l'Etoile, France), and the plates were incubated at 37 C for 48 h. Bacteria forming colonies were identified using Vitek® 2 Gram-negative Identification Cards (bioMérieux, Marcy l'Etoile, France) and studied for ESBL production as described above.