CORRELATION BETWEEN MITOCHONDRIAL-DNA 4977-BP DELETION AND RESPIRATORY-CHAIN ENZYME-ACTIVITIES IN AGING HUMAN SKELETAL-MUSCLES
CORRELATION BETWEEN MITOCHONDRIAL-DNA 4977-BP DELETION AND RESPIRATORY-CHAIN ENZYME-ACTIVITIES IN AGING HUMAN SKELETAL-MUSCLES
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DOI：
10.1006/bbrc.1994.2732
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发表时间：
1994-11-30
影响因子：
3.1
通讯作者：
GADALETA, MN
中科院分区：
文献类型：
--
作者：
LEZZA, AMS;BOFFOLI, D;GADALETA, MN
对同一人骨骼肌标本进行线粒体DNA4977-BP缺失含量和呼吸链酶活性的测定。线粒体DNA损伤与生物能量缺乏之间存在直接关联。观察线粒体DNA缺失出现的时程。线粒体DNA缺失分子所占比例最高为0.26%，出现在80年代，与呼吸链酶活性主要降低的年龄相对应。两个线粒体呼吸酶活性很低的样本，与年龄相近的样本相比，缺失程度要高得多。然而，考虑到这些样本中缺失的绝对水平很低，我们认为对呼吸链复合体，特别是复合体IV的损伤可能是老年人骨骼肌线粒体DNA分子数量增加的更大原因。(C)1994年学术出版社。
The content of the mitochondrial DNA 4977-bp deletion and the respiratory chain enzyme activities were determined in the same human skeletal muscle specimens. A direct correlation between damage to mtDNA and bioenergetic deficiency was observed. The time-course of the appearance of the mtDNA deletion was followed. The highest percentage of mtDNA-deleted molecules was 0.26% and it was found in the eighties which corresponds to the age of the major reduction in the respiratory chain enzyme activities. Two samples with very low mitochondrial respiratory enzyme activities exhibited much higher levels of deletion compared to the similar age counterparts. Given, however, the low absolute level of the deletion also in these samples, we suggest that damage to the respiratory chain complexes, especially complex IV, might be the cause more than the effect of the increased number of mtDNA molecules bearing deletions in aged human skeletal muscle. (C) 1994 Academic Press, Inc.