Crenobacter caeni sp. nov. Isolated from Sludge
Crenobacter caeni sp. nov. Isolated from Sludge
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卡氏钝杆菌
DOI：
10.1007/s00284-020-02233-7
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发表时间：
2020
影响因子：
2.6
通讯作者：
Jian He
中科院分区：
文献类型：
--
作者：
Zhou Yang;Jianyi Xu;Mengyao Sheng;J. Qiu;Jian;Jun Zhang;Jian He
菌株HX-7-9T是从中国江苏省南京市某农业化工厂生化处理设施出口收集的活性污泥中分离得到的。菌株HX-7-9T革兰氏染色阴性，棒状，无孢子形成，鞭毛。基于16S rRNA基因的系统发育分析表明，菌株HX-7-9T属于Crenobacter属，与Crenobacter luteus ym - 78141t有中度亲缘关系（相似度为94.8%）。菌株基因组DNA G+C含量为67.5 mol%。菌株HX-7-9T能在16-45℃（37℃最适）、pH 6.0-8.0 （pH 7.0最适）和0 - 1% (w/v) NaCl（0最适）条件下生长。脂肪酸主要成分为C16:0和特征3 （C16:1ω7c和/或C16:1ω6c）。呼吸泛素Q-8。极性脂质谱由二磷脂酰甘油（DPG）、磷脂酰单甲基乙醇胺（PME）、磷脂酰乙醇胺（PE）、磷脂（PL）、糖脂（GL）和氨基磷脂（APL）组成。HX-7-9T和黄体C. YIM-78141T基因组间的ANI和dDDH值分别为79.8和19.1%。根据表型、化学分类和基因型分析，菌株HX-7-9T为Crenobacter属的一个新种，命名为Crenobacter caeni sp. 11。型应变为HX-7-9T （= KCTC 72654T = CCTCC AB 2019349T）。
Strain HX-7-9T was isolated from the activated sludge collected from the outlet of the biochemical treatment facility of agricultural chemical plant in Nanjing, Jiangsu province, PR China. Strain HX-7-9T is Gram staining-negative, rod-shaped, non-spore-forming and flagellated. The 16S rRNA gene-based phylogenetic analysis indicate that strain HX-7-9T belongs to the genus Crenobacter, moderately related to Crenobacter luteus YIM-78141T (94.8% similarity). The genomic DNA G+C content of the strain was 67.5 mol%. Strain HX-7-9T was able to grow at 16–45 °C (optimum at 37 °C), at pH 6.0–8.0 (optimum at pH 7.0) and with 0–1% (w/v) NaCl (optimum at 0). Predominant fatty acid constituents were C16:0 and summed feature 3 (C16:1ω7c and/or C16:1ω6c). The respiratory ubiquinone was Q-8. The polar lipid profile is composed of diphosphatidylglycerol (DPG), phosphatidylmonomethylethanolamine (PME), phosphatidylethanolamine (PE), phospholipids (PL), glycolipid (GL) and aminophospholipid (APL). The ANI and dDDH values obtained between the genomes of HX-7-9T and C. luteus YIM-78141T were 79.8 and 19.1%, respectively. On the basis of data from phenotypic, chemotaxonomic and genotypic analysis, strain HX-7-9T represents a novel species of the genus Crenobacter, for which the name Crenobacter caeni sp. nov. is proposed. The type strain is HX-7-9T (= KCTC 72654T = CCTCC AB 2019349T).
影响因子：
14.9
作者：
THOMPSON, JD;HIGGINS, DG;GIBSON, TJ
通讯作者：
GIBSON, TJ
隆重推出 EzBioCloud：16S rRNA 基因序列和全基因组组件的分类学统一数据库。
DOI：
10.1099/ijsem.0.001755
发表时间：
2017-05
影响因子：
2.8
作者：
Yoon SH;Ha SM;Kwon S;Lim J;Kim Y;Seo H;Chun J
通讯作者：
Chun J