CDNA ISOLATED FROM A HUMAN T-CELL LIBRARY ENCODES A MEMBER OF THE PROTEIN-TYROSINE-PHOSPHATASE FAMILY
CDNA ISOLATED FROM A HUMAN T-CELL LIBRARY ENCODES A MEMBER OF THE PROTEIN-TYROSINE-PHOSPHATASE FAMILY
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DOI：
10.1073/pnas.86.14.5257
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发表时间：
1989-07-01
影响因子：
11.1
通讯作者：
KREBS, EG
中科院分区：
文献类型：
--
作者：
COOL, DE;TONKS, NK;KREBS, EG
用两种标记的合成寡核苷酸编码人胎盘蛋白酪氨酸磷酸酶（蛋白酪氨酸磷酸磷酸水解酶，EC 3.1.3.48）的区域筛选人外周T细胞cDNA文库。分离出一个阳性克隆并测定其核苷酸序列。它含有1305个碱基对的开放阅读框和一个TAA终止密码子，978个碱基对的3“非翻译端，但没有发现poly（A）+尾，在61位预测有一个起始的甲硫氨酸残基，这将产生一个415个氨基酸残基的蛋白质（Mr，48，400）。这得到了在体外网织红细胞裂解物翻译系统中使用从克隆的cDNA和T7 RNA聚合酶转录的RNA合成Mr 48，400蛋白的支持。推导的氨基酸序列与其他已知的蛋白质进行比较，揭示65%的同一性，从胎盘中分离的低Mr PTP1B。鉴于高度的相似性，T细胞cDNA可能编码一种新发现的蛋白酪氨酸磷酸酶，从而扩展了该基因家族。
A human peripheral T-cell cDNA library was screened with two labeled synthetic oligonucleotides encoding regions of a human placenta-protein-tyrosine-phosphatase (protein-tyrosine-phosphate phosphohydrolase, EC 3.1.3.48). One positive clone was isolated and the nucleotide sequence was determined. It contained 1305 base pairs of open reading frame followed by a TAA stop codon and 978 base pairs of 3'' untranslated end, although a poly(A)+ tail was not found. An initiator methionine residue was predicted at position 61, which would result in a protein of 415 amino acid residues (Mr, 48,400). This was supported by the synthesis of a Mr 48,400 protein in an in vitro reticulocyte lysate translation system using RNA transcribed from the cloned cDNA and T7 RNA polymerase. The deduced amino acid sequence was compared to other known proteins revealing 65% identity to the low Mr PTPase 1B isolated from placenta. In view of the high degree of similarity, the T-cell cDNA likely encodes a newly discovered protein-tyrosine-phosphatase, thus expanding this family of genes.