A Quantitative Assessment of Wound Healing With Oxygenated Micro/Nanobubbles in a Preclinical Burn Model.
A Quantitative Assessment of Wound Healing With Oxygenated Micro/Nanobubbles in a Preclinical Burn Model.
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DOI：
10.1097/sap.0000000000003017
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发表时间：
2021-10-01
影响因子：
1.5
通讯作者：
Widgerow AD
中科院分区：
文献类型：
--
作者：
Sayadi LR;Rowland R;Naides A;Tomlinson L;Ponticorvo A;Durkin AJ;Widgerow AD
烧伤是一种毁灭性的伤害，具有显著的发病率，需要长期治疗或多次重建手术。由烧伤创面转化引起的创面愈合和二次损伤可归因于治疗干预，其中缺血被认为是主要因素之一。在停滞区停止损伤进展是至关重要的，因为转化会增加烧伤表面积和深度，导致局部和全身性后果。载氧微/纳米气泡（MNB（O2））提供了一种新技术，可用于有效地将氧气输送到烧伤伤口，并可能抵消烧伤伤口缺血。在大鼠模型（n =3）上用MNB（O2）局部冲洗全层烧伤伤口与盐水处理的对照（n = 3）进行比较。通过空间频域成像（SFDI）和激光散斑成像（LSI）在28天的过程中定量组织结构（约化散射系数，μ s '）、氧合血红蛋白浓度（cHbO 2）和组织灌注。检查在实验结束时采集的组织学样品的伤口愈合证据。该初步研究的结果显示，在MNB（O2）组中，伤口愈合期间（第11-28天），SFDI测量的μs'具有显著差异，加速愈合。愈合“临界点”似乎发生在第9-11天，胶原组织增加，cHbO 2增加发生在该时间段附近，证实了观察到的总体愈合改善。此外，组织学证据表明，只有MNB（O2）烧伤在28天研究期结束时达到了重塑阶段。这些初步研究结果提出了MNB（O2）作为改善烧伤伤口愈合的局部方法的潜力。
Burns are devastating injuries, carry significant morbidity, and require long-term treatment or multiple reconstructive procedures. Wound healing and secondary insults caused by burn wound conversion is amendable to therapeutic intervention, where ischemia has been cited as one of the major factors. Halting injury progression in the zone of stasis is crucial as conversion creates increased burn surface area, depth, leading to local and systemic consequences. Oxygen carrying Micro/nanobubbles, (MNB(O2)), offer a novel technology which can be used to effectively deliver oxygen to burn wounds and potentially counteract burn wound ischemia. Topical irrigation with MNB(O2) of full-thickness burns wounds on a rat model (n=3) were compared against saline treated controls (n=3). Tissue structure (reduced scattering coefficient, μs’), oxyhemoglobin concentration (cHbO2), and tissue perfusion were quantified over the course of 28 days through spatial frequency domain imaging (SFDI) and laser speckle imaging (LSI). Histological samples taken at the end of the experiment were examined for evidence of wound healing. Findings in this preliminary study showed hastened healing with significant differences in SFDI-measured μs’ during wound healing (days 11-28) in MNB(O2) group. The healing ‘tipping point’ appeared to occur at days 9-11 with increased collagen organization, increased cHbO2 occurring around that period confirming the gross healing improvements observed. In addition, histological evidence indicated that only the MNB(O2) burns had reached the remodeling phase by the end of 28-day study period. These preliminary findings propose the potential of MNB(O2) as a topical method for improving burn wound healing.