Recent advances in enzyme inhibition based-electrochemical biosensors for pharmaceutical and environmental analysis
Recent advances in enzyme inhibition based-electrochemical biosensors for pharmaceutical and environmental analysis
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DOI：
10.1016/j.talanta.2022.124092
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发表时间：
2023-02
期刊：
影响因子：
6.1
通讯作者：
Jingrong Li;Hongen Chang;Nan Zhang;Yongzhi He;Di Zhang;Boshi Liu;Yuxin Fang
中科院分区：
文献类型：
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作者：
Jingrong Li;Hongen Chang;Nan Zhang;Yongzhi He;Di Zhang;Boshi Liu;Yuxin Fang
酶是一种生物催化剂，能以高度特异和高效的方式促进各种生化反应。开发几种临床相关酶的抑制剂，包括乙酰胆碱酯酶、酪氨酸酶、黄嘌呤氧化酶、碱性磷酸酶和葡萄糖苷酶，已成为治疗多种疾病的一种有前途的方法。无意中摄入杀虫剂或其他有毒化合物也可能可逆或不可逆地抑制某些酶的活性，损害受影响个人的正常生理活动。因此，迫切需要设计可靠的方法来检测一系列样品基质中酶抑制剂的存在。电化学生物传感器具有成本低、效率高、灵敏度高等特点，在酶抑制剂的开发和检测中具有广阔的应用前景。本文综述了基于酶抑制的电化学生物传感器的研究现状。在初步讨论了在设计这些生物传感器时采用的不同类型的抑制和固定化策略之后，重点讨论了基于酶的电化学生物传感器的设计的研究进展，目的是在药物开发和环境污染的背景下检测酶抑制剂。最后，本文总结了与电化学生物传感器制造相关的当前挑战和未来发展趋势。综上所述，这一综述将为未来专注于创造用于相关领域的新型电化学生物传感器的研究和开发工作提供坚实的基础。
Enzymes are biological catalysts that facilitate a diverse range of biochemical reactions in a highly specific and efficient manner. The development of inhibitors of several clinically relevant enzymes including acetylcholinesterase, tyrosinase, xanthine oxidase, alkaline phosphatase, and glucosidase has emerged as a promising approach to treating several diseases. The unintentional intake of pesticides or other toxic compounds can also reversibly or irreversibly inhibit the activity of certain enzymes, damaging normal physiological activity in affected individuals. There is thus a critical need to design reliable approaches to detecting the presence of enzymatic inhibitors in a range of sample matrices. Electrochemical biosensors have emerged as promising tools that have been widely employed in the development and detection of enzymatic inhibitors owing to their low-cost, highly efficient, and extremely sensitive characteristics. The present review provides a comprehensive overview of the current status of enzyme inhibition-based electrochemical biosensors. After an initial discussion of different types of inhibition and immobilization strategies employed in the design of these biosensors, the current progress of research efforts focused on the design of enzyme-based electrochemical biosensors aimed at detecting enzymatic inhibitors in the context of both drug development and environmental pollution is discussed. Lastly, this article closes with an overview of current challenges and future development trends associated with electrochemical biosensor fabrication efforts. Together, this review will provide a robust foundation for future research and development work focused on creating novel electrochemical biosensors for use in related fields.