Postprandial modulation of serum paraoxonase activity and concentration in diabetic and non-diabetic subjects
Postprandial modulation of serum paraoxonase activity and concentration in diabetic and non-diabetic subjects
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DOI：
10.1016/j.numecd.2005.09.005
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发表时间：
2006-10-01
影响因子：
3.9
通讯作者：
James, Richard W.
中科院分区：
文献类型：
--
作者：
Beer, Sandra;Moren, Xenia;James, Richard W.
目的：分析HDL相关的抗氧化酶对氧磷酶-1，在餐后hyperlipaemia.Methods和结果：2型糖尿病患者（n = 72），葡萄糖不耐受患者（n = 10）和对照组（n = 38）消耗高脂肪：高碳水化合物餐。收集血液样本长达4小时，并分析脂质和对氧磷酶-1。体外研究检查了HDL对酶的功能。餐后血清甘油三酯显著升高。对氧磷酶-1活性在整个餐后阶段显著降低。酶的浓度最初显著降低，但在4 h时恢复至空腹浓度。与禁食相比，4小时时的比活性显著降低。比活度的降低与餐后动态相有关。脂蛋白代谢载脂蛋白A1限制对氧磷酶-1的损失。HDL在体外餐后条件下与PON 1结合和稳定的能力降低。结论：餐后高脂血症与血清对氧磷酶-1的变化有关，与HDL抗氧化能力降低一致。在糖尿病和非糖尿病患者之间没有观察到差异，表明该效应与餐后高脂血症有关。对氧磷酶-1的修饰可能导致与餐后脂血症相关的血管疾病风险增加，特别是在血清对氧磷酶-1已经缺乏的糖尿病患者中。（C）2005 Elsevier B. V.保留所有权利。
Objectives: To analyse the HDL associated anti-oxidant enzyme paraoxonase-1, during postprandial hyperlipaemia.Methods and results: Type 2 diabetic patients (n = 72), glucose intolerant patients (n = 10) and controls (n = 38) consumed a high fat:high carbohydrate meal. Blood samples were collected up to 4 h and analysed for lipids and paraoxonase-1. In vitro studies examined HDL function with respect to the enzyme. There were significant postprandial increases in serum triglycerides. Paraoxonase-1 activity decreased significantly throughout the postprandial phase. Concentrations of the enzyme initially decreased significantly, but returned to fasting concentrations at 4 h. Specific activities were significantly lower at 4 h, compared to fasting. The decrease in specific activity was linked to the dynamic phase of postprandial. lipoprotein metabolism. Apo Al limited loss of paraoxonase-1. HDL isolated after being subjected to post-prandial conditions in vitro had reduced capacity to associate with and stabilise PON1.Conclusions: Postprandial hyperlipaemia was associated with changes to serum paraoxonase-1, consistent with a reduced anti-oxidant potential of HDL. No differences were observed between diabetic and non-diabetic patients, suggesting that the effect was linked to postprandial hyperlipaemia. Modifications to paraoxonase-1 could contribute to increased risk of vascular disease associated with postprandial lipaemia, particularly in diabetic patients, who are already deficient in serum paraoxonase-1. (C)2005 Elsevier B.V. All rights reserved.