Involvement of the c-Ski oncoprotein in cell cycle arrest and transformation during nurse cell formation after Trichinella spiralis infection
Involvement of the c-Ski oncoprotein in cell cycle arrest and transformation during nurse cell formation after Trichinella spiralis infection
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DOI：
10.1016/j.ijpara.2006.05.012
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发表时间：
2006-09-01
影响因子：
4
通讯作者：
Takahashi, Y.
中科院分区：
文献类型：
--
作者：
Wu, Z.;Nagano, I.;Takahashi, Y.
通过跟踪c-Ski（蛋白质和mRNA）在受感染肌肉细胞中的表达动力学和分布，以及与c-Ski配合的转化生长因子β（TGF-β）信号通路因子基因（TGF-β、Smad2和Smad4）的表达动力学，研究了c-Ski（由癌基因c-ski编码的癌蛋白）在旋毛虫感染的肌肉组织中在护士细胞形成过程中的作用。使用抗 c-Ski 抗体进行的免疫组织化学分析表明，在感染早期（感染后 13 天和 18 天 (p.i.)），c-Ski 蛋白表达的增加仅限于嗜酸性细胞质，而不是增大的细胞核或嗜碱性细胞质。在感染后期（注射后 23 和 28 天），c-Ski 蛋白仅限于嗜碱性细胞质中增大的细胞核，而不是嗜酸性细胞质。注射后 48 天时，几乎检测不到 c-Ski 蛋白。实时PCR分析显示，c-ski基因的表达从接种后13天开始增加，在接种后23-28天达到峰值。然后到 48 天后降至低水平。 TGF-β 信号通路因子基因（TGF-β、Smad2 和 Smad4）的表达动力学与 c-ski 相似。这些发现提供了证据，表明 c-Ski 蛋白通过宿主细胞核中的 TGF-β 信号通路过程参与滋养细胞的形成。 (c) 2006 年由 Elsevier Ltd 代表澳大利亚寄生虫学协会出版。
The role of c-Ski, an oncoprotein encoded by the oncogene, c-ski, in Trichinella spiralis-infected muscle tissues during nurse cell formation, was investigated by following the expression kinetics and distribution of c-Ski (both protein and mRNA) in the infected muscle cell, as well as the expression kinetics of the transforming growth factor beta (TGF-beta) signaling pathway factor genes (TGF-beta, Smad2 and Smad4) which cooperate with c-Ski. Immunohistochemical analysis using an anti-c-Ski antibody indicated that in the early stages of infection (13 and 18 days post-infection (p.i.)) the increased expression of the c-Ski protein was limited to the eosinophilic cytoplasm and not the enlarged nuclei or basophilic cytoplasm. At a later stage of infection (23 and 28 days p.i.) the c-Ski protein was limited to the enlarged nuclei in the basophilic cytoplasm, rather than the eosinophilic cytoplasm. At 48 days p.i., the c-Ski protein was barely detectable. Real-time PCR analysis showed that expression of the c-ski gene increased from 13 days p.i., reached a peak at 23-28 days p.i. and then decreased to a low level by 48 days p.i. Expression kinetics for the TGF-beta signaling pathway factor genes (TGF-beta, Smad2 and Smad4) were similar to that of c-ski. These findings provide evidence that the c-Ski protein is involved in nurse cell formation through the TGF-beta signaling pathway process in the host cell nucleus. (c) 2006 Published by Elsevier Ltd on behalf of Australian Society for Parasitology Inc.