The Bone Regeneration Model and Primary Osteoblastic Cell Culture Used in the Analysis of Ccn3 Transgenic and Knockout Mice
The Bone Regeneration Model and Primary Osteoblastic Cell Culture Used in the Analysis of Ccn3 Transgenic and Knockout Mice
复制标题
用于 Ccn3 转基因和敲除小鼠分析的骨再生模型和原代成骨细胞培养
DOI：
10.1007/978-1-4939-6430-7_27
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Yamaguchi A.
中科院分区：
文献类型：
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作者：
Sakamoto K;Matsushita Y;Minamizato T;Katsuki Y;Katsube K;Yamaguchi A.
骨组织本质上是硬的，因此，它比软组织更难处理、加工和检查。在这里，我们描述了一个实验模型的骨再生和几个选定的协议，用于调查骨中的mRNA和蛋白质表达。CCN 3对膜性骨形成的抑制功能已经通过遵循本文所述的方案得到证实（图1）。
Bone tissue is intrinsically hard and thus, it is more difficult to handle, process, and examine than soft tissues. Here, we describe an experimental model of bone regeneration and several selected protocols useful for investigating mRNA and protein expression in bone. The inhibitory function of CCN3 on membranous bone formation has been confirmed by following the protocols described herein (Fig. 1).