NK cells influence both innate and adaptive immune responses after mucosal immunization with antigen and mucosal adjuvant.
NK cells influence both innate and adaptive immune responses after mucosal immunization with antigen and mucosal adjuvant.
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DOI：
10.4049/jimmunol.0903357
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发表时间：
2010-04-15
期刊：
影响因子：
--
通讯作者：
Dougan G
中科院分区：
文献类型：
--
作者：
Hall LJ;Clare S;Dougan G
用结核分枝杆菌的Ag85B-ESAT6抗原混合大肠杆菌热不稳定毒素作为佐剂经鼻免疫小鼠后，发现NK细胞以一种暂时可控的方式被募集到鼻相关淋巴组织和颈部淋巴结。这些NK细胞被激活并分泌多种细胞因子和其他免疫调节剂。利用靶向抗亚洲草GM1的抗体消耗，我们发现与对照动物相比，免疫NK细胞耗尽小鼠的树突状细胞、巨噬细胞和中性粒细胞的运输、活化和细胞因子分泌发生改变的证据。抗原特异性免疫反应分析显示，免疫NK细胞耗尽动物的抗体和细胞因子反应减弱。全身给药rIL-6而非rIFN-γ可显著恢复NK细胞缺失小鼠的免疫应答。综上所述，细胞因子的产生，特别是IL-6，通过NK细胞和NK细胞激活的免疫群体，在粘膜免疫后局部先天免疫应答的建立和随之而来的适应性免疫的发展中起着重要作用。
NK cells were found to be recruited in a temporally controlled manner to the nasal-associated lymphoid tissue and the cervical lymph nodes of mice following intranasal immunisation with Ag85B-ESAT6 antigen from Mycobacterium tuberculosis mixed with Escherichia coli heat-labile toxin as adjuvant. These NK cells were activated and they secreted a diverse range of cytokines and other immunmodulators. Using antibody depletion targeting anti-asialo GM1, we found evidence for altered trafficking, impaired activation and cytokine secretion of dendritic cells, macrophages and neutrophils in immunised NK cell depleted mice compared to control animals. Analysis of antigen-specific immune responses revealed an attenuated antibody and cytokine response in immunised NK cell depleted animals. Systemic administration of rIL-6 but not rIFN-γ significantly restored immune responses in mice depleted of NK cells. In conclusion, cytokine production, particularly IL-6, via NK cells and NK cell activated immune populations, plays an important role in the establishment of local innate immune responses and the consequent development of adaptive immunity after mucosal immunisation.
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影响因子：
64.8
作者：
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通讯作者：
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影响因子：
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