Use of multiple colorimetric indicators for paper-based microfluidic devices
Use of multiple colorimetric indicators for paper-based microfluidic devices
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DOI：
10.1016/j.aca.2010.06.019
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发表时间：
2010-08-03
影响因子：
6.2
通讯作者：
Henry, Charles S.
中科院分区：
文献类型：
--
作者：
Dungchai, Wijitar;Chailapakul, Orawon;Henry, Charles S.
我们在这里报告了纸基微流体装置 (mu PAD) 对单一分析物使用多个指示剂，以提高视觉区分分析物浓度的能力。在现有的 mu PAD 中，使用单一染料系统来测量单一分析物。在我们的方法中，设备被设计为使用每种分析物的多个指示剂同时量化分析物，从而提高测定的准确性。对单一分析物使用多种指示剂可以在不同的分析物浓度范围内产生不同的指示剂颜色，并提高更好地视觉区分颜色的能力。我们设备的原理是基于每种分析物特有的氧化酶产生的过氧化氢对指示剂的氧化。每种指示剂在不同的过氧化物浓度和分析物浓度下发生反应，从而扩大了操作范围。为了证明我们方法的实用性，选择 4-氨基安替比林和 3,5-二氯-2-羟基苯磺酸、邻二茴香胺二盐酸盐、碘化钾、酸性黑和酸性黄的混合物作为指示剂，在 1,,PAD 上同时半定量测量葡萄糖、乳酸和尿酸。我们的方法已成功应用于量化临床相关范围内的葡萄糖 (0.5-20 mM)、乳酸 (1-25 mM) 和尿酸 (0.1-7 mM)。还对对照血清和尿液样品中的葡萄糖、乳酸和尿酸进行了测定，以证明该装置对于生物样品分析的适用性。最后，使用未经训练的读者对多指标和单指标系统的结果进行了比较，以证明新系统所实现的准确性的提高。 (C) 2010 Elsevier B.V. 保留所有权利。
We report here the use of multiple indicators for a single analyte for paper-based microfluidic devices (mu PAD) in an effort to improve the ability to visually discriminate between analyte concentrations. In existing mu PADs, a single dye system is used for the measurement of a single analyte. In our approach, devices are designed to simultaneously quantify analytes using multiple indicators for each analyte improving the accuracy of the assay. The use of multiple indicators for a single analyte allows for different indicator colors to be generated at different analyte concentration ranges as well as increasing the ability to better visually discriminate colors. The principle of our devices is based on the oxidation of indicators by hydrogen peroxide produced by oxidase enzymes specific for each analyte. Each indicator reacts at different peroxide concentrations and therefore analyte concentrations, giving an extended range of operation. To demonstrate the utility of our approach, the mixture of 4-aminoantipyrine and 3,5-dichloro-2-hydroxy-benzenesulfonic acid, o-dianisidine dihydrochloride, potassium iodide, acid black, and acid yellow were chosen as the indicators for simultaneous semi-quantitative measurement of glucose, lactate, and uric acid on a 1,,PAD. Our approach was successfully applied to quantify glucose (0.5-20 mM), lactate (1-25 mM), and uric acid (0.1-7 mM) in clinically relevant ranges. The determination of glucose, lactate, and uric acid in control serum and urine samples was also performed to demonstrate the applicability of this device for biological sample analysis. Finally results for the multi-indicator and single indicator system were compared using untrained readers to demonstrate the improvements in accuracy achieved with the new system. (C) 2010 Elsevier B.V. All rights reserved.