A single packet of transmitter does not saturate postsynaptic glutamate receptors
A single packet of transmitter does not saturate postsynaptic glutamate receptors
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DOI：
10.1016/s0896-6273(02)00692-x
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发表时间：
2002-05-16
期刊：
影响因子：
16.2
通讯作者：
Takahashi, T
中科院分区：
文献类型：
--
作者：
Ishikawa, T;Sahara, Y;Takahashi, T
神经递质储存在突触囊泡中，并通过胞吐作用释放到突触间隙中。中枢突触传递的基本问题之一是递质的量子包是否饱和突触后受体。为了解决这个问题，我们通过全细胞记录移液管将兴奋性递质L-谷氨酸加载到大鼠脑干切片中的巨大神经末梢，即Held萼。这引起由α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）或N-甲基-D-天冬氨酸（NMDA）受体介导的量子和动作电位诱发的EPSC的显著增强。这些结果直接表明，AMPA和NMDA受体都不是饱和的单包的发射器，并表明囊泡发射器的内容是一个重要的决定因素的突触效力。
Neurotransmitter is stored in synaptic vesicles and released by exocytosis into the synaptic cleft. One of the fundamental questions in central synaptic transmission is whether a quantal packet of transmitter saturates postsynaptic receptors. To address this question, we loaded the excitatory transmitter L-glutamate via whole-cell recording pipettes into the giant nerve terminal, the calyx of Held, in rat brainstem slices. This caused marked potentiations of both quantal and action potential-evoked EPSCs mediated by alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) or N-methyl-D-aspartate (NMDA) receptors. These results directly demonstrate that neither AMPA nor NMDA receptors are saturated by a single packet of transmitter, and indicate that vesicular transmitter content is an important determinant of synaptic efficacy.