Plasma membrane lipid organization and the adherence of differentiating lymphocytes to macrophages.
Plasma membrane lipid organization and the adherence of differentiating lymphocytes to macrophages.
复制标题
质膜脂质组织和分化淋巴细胞与巨噬细胞的粘附。
DOI：
10.1002/jcp.1041380110
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发表时间：
1989
影响因子：
5.6
通讯作者：
Schlegel,RA
中科院分区：
文献类型：
--
作者：
DelBuono,BJ;White,SM;Williamson,PL;Schlegel,RA
在原发性和继发性淋巴器官分化过程中，淋巴细胞质膜磷脂的包装发生变化。在分化过程中，淋巴细胞与包括巨噬细胞在内的多种网状内皮细胞相互作用。为了研究这两种现象之间的可能关系，我们在体外测量了淋巴细胞和巨噬细胞之间相互作用的强度，作为淋巴细胞表面磷脂包装紧密程度的函数。粘附强度是通过淋巴细胞在离心力增加时保持对巨噬细胞粘附的能力来测量的。使用荧光亲脂探针merocyanine 540 （MC540）评估磷脂堆积，该探针优先结合脂质堆积更松散的双层。小鼠胸腺细胞的三个亚群分别与腹膜或胸腺巨噬细胞的粘附强度有关。为了确定这些亚群是否与MC540荧光可区分的三类细胞相对应，我们检测了染色或非染色细胞的富集群体，以及根据MC540荧光强度分选的细胞。荧光最小的细胞贴壁强度最小；荧光细胞越多，黏附力越强；中等荧光细胞具有中等粘附性。当检查脾淋巴细胞与腹膜或脾巨噬细胞的粘附性时，可以看到类似的荧光和粘附模式。这些结果表明，淋巴细胞的质膜脂双分子层的组织可能参与了它们在原发性和继发性分化过程中与巨噬细胞的相互作用。因此，淋巴细胞的粘附信号可能类似于其他血细胞的粘附信号。
Changes in the packing of phospholipids in the plasma membrane of lymphocytes occur during differentiation within primary and secondary lymphoid organs. As they differentiate, lymphocytes interact with a variety of reticuloendothelial cells, including macrophages. To investigate a possible relation between these two phenomena, the strength of the interactions between lymphocytes and macrophages was measured in vitro as a function of the tightness of packing of phospholipids on the lymphocyte surface. Strength of adherence was measured by the ability of lymphocytes to remain adherent to macrophages when subjected to increasing centrifugal forces. Phospholipid packing was assessed using the fluorescent lipophilic probe merocyanine 540 (MC540), which preferentially binds to bilayers in which the lipids are more loosely packed. Three subpopulations of murine thymocytes were resolved with respect to strength of adherence to peritoneal or thymic macrophages. To determine whether these subpopulations corresponded with the three classes of cells distinguishable by MC540 fluorescence, populations enriched for staining or non‐staining cells, and cells sorted on the basis of MC540 fluorescence intensity, were examined. The least fluorescent cells were the least strongly adherent; the most fluorescent cells were the most strongly adherent; and cells of intermediate fluorescence had intermediate adherence. When splenic lymphocytes were examined with respect to adherence to peritoneal or splenic macrophages, similar patterns of fluorescence and adherence were seen. These results suggest that the organization of the plasma membrane lipid bilayer of lymphocytes may be involved in their interactions with macrophages during primary and secondary differentiation. The adherence signal for lymphocytes thus may be similar to that proposed for other blood cells.