CYCLIC-AMP AND ADENYLATE-CYCLASE ACTIVATORS STIMULATE TRYPANOSOMA-CRUZI DIFFERENTIATION
CYCLIC-AMP AND ADENYLATE-CYCLASE ACTIVATORS STIMULATE TRYPANOSOMA-CRUZI DIFFERENTIATION
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DOI：
10.1016/0014-4894(88)90092-6
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发表时间：
1988-08-01
影响因子：
2.1
通讯作者：
GOLDENBERG, S
中科院分区：
文献类型：
--
作者：
GONZALESPERDOMO, M;ROMERO, P;GOLDENBERG, S
采用化学方法确定体外分化条件，研究环磷酸腺苷(CAMP)和腺苷环化酶激活剂在克氏锥虫上鞭毛体向感染性后环类鞭毛体转化(后生)过程中的作用。观察到在分化培养基中加入cAMP类似物或腺苷环化酶激活剂都能刺激上鞭毛体向亚环鞭毛体的转化。这些结果进一步得到证实，cAMP磷酸二酯酶的抑制剂具有刺激性，而该酶的激活剂则抑制化生过程。另一方面，钙调蛋白的抑制剂抑制了上鞭毛体向亚环鞭毛体的转化，这表明克氏锥虫的腺苷酸环化酶可能被钙调蛋白激活。此外，这些结果强烈表明鸟嘌呤核苷酸结合蛋白参与了克鲁氏毛滴虫腺苷环化酶的激活。该系统可用于研究真核生物的细胞分化机制。
A chemically defined in vitro differentiating condition was used to study the potential role of cyclic AMP (cAMP) and adenylate cyclase activators on the transformation of Trypanosoma cruzi epimastigotes to the infective metacyclic trypomastigotes (metacyclogenesis). It was observed that both addition of cAMP analogs or adenylate cyclase activators to the differentiating medium stimulated the transformation of epimastigotes to metacyclic trypomastigotes. These results were further corroborated by showing that inhibitors of cAMP phosphodiesterase were stimulatory while activators of this enzyme inhibited the metacyclogenesis process. On the other hand, inhibitors of calmodulin inhibited the transformation of epimastigotes to metacyclic trypomastigotes, suggesting that T. cruzi adenylate cyclase might be activated by calmodulin. In addition, the results strongly suggest that guanine nucleotide binding proteins are involved in T. cruzi adenylate cyclase activation. This system may be useful for studying cell differentiation mechanisms in eukaryotes.