Immunophenotypic and cytogenetic comparison of Waldenstrom's macroglobulinemia with splenic marginal zone lymphoma
Immunophenotypic and cytogenetic comparison of Waldenstrom's macroglobulinemia with splenic marginal zone lymphoma
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DOI：
10.3816/clm.2005.n.007
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发表时间：
2005-03-01
期刊：
影响因子：
--
通讯作者：
San Miguel, JF
中科院分区：
文献类型：
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作者：
Ocio, EM;Hernández, JM;San Miguel, JF
某些B细胞淋巴增生性疾病显示血清单克隆免疫球蛋白（IG）M蛋白，可能难以与瓦尔登斯特伦巨球蛋白血症（WM）鉴别。本文报告了85例WM的免疫表型和细胞遗传学特点，并与29例脾边缘区淋巴瘤（SMZL）进行了比较。对于免疫表型，WM和SMZL持续表达泛B细胞标志物（CD 19、CD 20、CD 22和表面IG）。然而，在kappa/lambda比值（SMZL为1.2：1，WM为4.51）和一些标志物（如CD 22和CD 11 c）方面存在差异，SMZL患者与WM患者相比，CD 22和CD 11 c过度表达，而CD 25在WM中更常见（88% vs. 44%）。WM中CD 103抗原均为阴性。SMZL组阳性率为40%。单克隆抗体FMC 7通常在两种实体中均为阳性：WM为异质性，而SMZL为同质性。CD 25和CD 22联合检测可鉴别WM和SMZL。WM中主要的分子异常是6 q缺失（在我们的经验中为30%），而SMZL中最常见的异常是沿着+3q（19%）和+5q（10%）的7 q缺失。有趣的是，免疫球蛋白重排的发生率低WM（12%）和SMZL（10%）。免疫表型和分子细胞遗传学研究有助于鉴别WM和SMZL。
Some B-cell lymphoproliferative disorders displaying a serum monoclonal immunoglobulin (Ig) M protein could be difficult to differentiate from Waldenstrom's macroglobulinemia (WM). We report on the immunophenotypic and cytogenetic characteristics of 85 patients With WM and compare them with 29 patients with splenic marginal zone lymphoma (SMZL). For immunophenotyping, WM and SMZL constantly expressed pan-B-cell markers (CD19, CD20, CD22, and surface Ig). However, there were differences in the kappa/lambda, ratio (1.2:1 for SMZL and 4.51 for WM) and in some markers such as CD22 and CD11c, which were overexpressed in patients with SMZL compared with patients with WM, whereas CD25 was more frequently positive in WM (88% vs. 44%). The CD103 antigen was always negative in WM. whereas it was positive in 40% of SMZL cases. The monoclonal antibody FMC7 was usually positive in both entities: heterogeneous in WM but homogeneous in SMZL. The combination of CD25 and CD22 could differentiate between WM and SMZL. The principal molecular abnormality in WM is 6q deletion (30% in our experience), whereas in SMZL the most common abnormalities are loss of 7q (19%) along with +3q (19%) and +5q (10%). Interestingly, the incidence of IgH rearrangement was low in WM (12%) and SMZL (10%). Immunophenotypic and molecular cytogenetic studies could help to distinguish WM from SMZL.