The path that RNA takes from the nucleus to the cytoplasm: a trip with some surprises
The path that RNA takes from the nucleus to the cytoplasm: a trip with some surprises
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DOI：
10.1007/s00418-002-411z
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发表时间：
2002-08-01
影响因子：
2.3
通讯作者：
Iborra, FJ
中科院分区：
文献类型：
--
作者：
Iborra, FJ
现在很清楚，核环境在基因表达中起着至关重要的作用。为此，我们开发了原位研究基因表达的方法。转录发生在哺乳动物细胞核质中的离散病灶中。这些位点集中了几种RNA聚合酶II和参与mRNA产生的因子。此外，这些位点还包含进行蛋白质合成的活性机制。一旦mRNA离开转录位点，它与核孔复合体相互作用，mRNA使用核孔复合体的细丝输出到核孔复合体的中间部分，最后释放到细胞质中。
It is now clear that nuclear context is playing an essential role in gene expression. For this reason we have developed, methods to study gene expression in situ. Transcription takes place in discrete foci in the nucleoplasm of mammalian cells. These sites concentrate several RNA polymerases II and factors involved in the production of mRNA. Moreover, these sites also contain the active machinery to carry out protein synthesis. Once the mRNA leaves the transcription site, it interacts with the nuclear pore complex and the mRNA is exported using the filaments of the nuclear pore complex in to the middle part of the nuclear pore complex and finally is released in the cytoplasm.