Inflammatory infiltrate of chronic periradicular lesions: an immunohistochemical study
Inflammatory infiltrate of chronic periradicular lesions: an immunohistochemical study
复制标题
DOI：
10.1046/j.1365-2591.2003.00627.x
复制
发表时间：
2003-07-01
影响因子：
5
通讯作者：
Rontogianni, D
中科院分区：
文献类型：
--
作者：
Liapatas, S;Nakou, M;Rontogianni, D
目的应用免疫组织化学方法测定人慢性根周病变的细胞谱，探讨根周肉芽肿和囊肿滤液中细胞的差异。研究人群包括45名无全身性疾病的个体。神经根周围手术期间行活检。石蜡包埋切片采用亲和素-生物素复合物法（ABC）染色，低温冷冻组织切片采用碱性磷酸酶抗碱性磷酸酶法（apap）染色。这些方法是高度有效和敏感的，使用一组特异性单克隆抗体：CD4， CD8, CD3, CD10， HLADR, CD20, CD45RO， CD68和CD57。这45个标本采用了这两种技术。结果病理诊断为：根周肉芽肿25例，根周囊肿17例，瘢痕组织3例。根周肉芽肿与囊肿的滤液炎症性差异无统计学意义。切片观察显示，炎性细胞以T淋巴细胞、B淋巴细胞和巨噬细胞为主。T淋巴细胞和B淋巴细胞在60%的病例中均匀分布。T-4/T-8比值约为1 ~ 3甚至更大，与根周组织炎症一致。B淋巴细胞向浆细胞的最终分化也被检测到，而自然杀伤细胞（NK）仅在10例（22%）中被发现。此外，抗原呈递细胞和T抑制细胞/细胞毒性细胞被发现与原有和新形成的上皮有关。结论根周肉芽肿和囊肿是慢性根周病变发展的两个不同阶段，是免疫反应过程不可抑制的正常结果。
Aim To determine the cellular profile of human chronic periradicular lesions using immunohistochemical methods in order to study the differences in the cell in filtrate of periradicular granulomas and cysts.Methodology The study population consisted of 45 individuals without any systemic disease. Biopsies were obtained during periradicular surgery. Paraffin-embedded sections were stained by the avidin-biotin complex method (ABC), whilst cryostat tissue sections were stained using the alkaline phosphatase antialkaline phosphatase assay (APAAP). These methods are highly valid and sensitive using a panel of specific monoclonal antibodies: CD4, CD8, CD3, CD10, HLADR, CD20, CD45RO, CD68 and CD57. The 45 specimens were characterized by the use of both techniques.Results The 45 specimens were histologically diagnosed as: 25 periradicular granulomas, 17 periradicular cysts and 3 scar tissues. No statistically significant differences were detected in the inflammatory in filtrate between periradicular granulomas and cysts. Observation of the sections showed that the majority of inflammatory cells consisted of T and B lymphocytes and macrophages. T and B lymphocytes were equally distributed in 60% of the cases. The T-4/T-8 ratio ranged approximately from 1 to 3 and greater, being consistent with inflammation of periradicular tissues. The final differentiation of B lymphocytes to plasma cells was also detected, whilst natural killer (NK) cells were found in only 10 cases (22%). Moreover, antigen presenting cells and T suppressor/cytotoxic cells were found to be associated with both pre-existing and newly formed epithelium.Conclusions Periradicular granulomas and cysts represent two different stages in the development of chronic periradicular pathosis as a normal result of the process of immune reactions that cannot be inhibited.