Immunolocalization of Aquaporin 1, 5, and 9 in the Female Pig Reproductive System
Immunolocalization of Aquaporin 1, 5, and 9 in the Female Pig Reproductive System
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DOI：
10.1369/jhc.2008.952499
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发表时间：
2009-01-01
影响因子：
3.2
通讯作者：
Nielsen, Soren
中科院分区：
文献类型：
--
作者：
Skowronski, Mariusz T.;Kwon, Tae-Hwan;Nielsen, Soren
哺乳动物水通道蛋白（AQP）的13种亚型已被鉴定，它们具有独特的组织特异性表达模式。已证实AQP存在于男性和女性人类、大鼠和小鼠的生殖系统中。然而，组织表达和细胞和亚细胞定位的水通道蛋白是未知的，在女性生殖系统的猪。在这项研究中，AQP 1免疫反应性检测卵巢毛细血管内皮细胞。在输卵管和子宫中也观察到毛细血管内皮的明显免疫标记。AQP 5在原始卵泡的扁平卵泡细胞、发育卵泡的颗粒细胞以及输卵管和子宫的肌细胞中表达。在输卵管上皮细胞和子宫上皮细胞中也观察到AQP 5的染色。AQP 9免疫反应在发育卵泡的颗粒细胞中观察到。AQP 9还定位于输卵管腔上皮细胞和子宫上皮细胞。据我们所知，这是第一个研究表明，组织表达和细胞和亚细胞定位的水通道蛋白在雌性猪的生殖系统。此外，这些结果表明，AQPs的几个亚型（AQP 1，5和9）参与调节水稳态在母猪的生殖系统。（J Histochem Cytochem 57：61-67，2009）
Thirteen mammalian aquaporin (AQP) isoforms have been identified, and they have a unique tissue-specific pattern of expression. AQPs have been documented in the reproductive system of both male and female humans, rats, and mice. However, tissue expression and cellular and subcellular localization of AQPs are unknown in the female reproductive system of pigs. In this study, AQP1 immunoreactivity was detected in the capillary endothelium of the ovary. Distinct immunolabeling of capillary endothelium was also observed in the oviduct and uterus. AQP5 was expressed in flattened follicle cells of primordial follicles, granulosa cells of developing ovarian follicles, and muscle cells of the oviduct and uterus. Staining of AQP5 was also observed in the epithelial cells of the oviduct and uterine epithelium. AQP9 immunoreactivity was observed in granulosa cells of developing follicles. AQP9 was also localized in the luminal epithelial cells of the oviduct and uterine epithelia cells. This is, to our knowledge, the first study that shows tissue expression and cellular and subcellular localization of AQPs in the reproductive system of the female pig. Moreover, these results suggest that several subtypes of the AQPs (AQP1, 5, and 9) are involved in regulation of water homeostasis in the reproductive system of gilts. (J Histochem Cytochem 57:61-67, 2009)