DISTRIBUTION OF ALCOHOL AND SORBITOL DEHYDROGENASES - ASSESSMENT OF MESSENGER-RNA SPECIES IN MAMMALIAN-TISSUES
DISTRIBUTION OF ALCOHOL AND SORBITOL DEHYDROGENASES - ASSESSMENT OF MESSENGER-RNA SPECIES IN MAMMALIAN-TISSUES
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DOI：
10.1111/j.1432-1033.1993.tb18059.x
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发表时间：
1993-07-15
期刊：
影响因子：
--
通讯作者：
HOOG, JO
中科院分区：
文献类型：
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作者：
ESTONIUS, M;DANIELSSON, O;HOOG, JO
研究了I、II和III类醇脱氢酶和山梨醇脱氢酶mRNA的组织分布。 纯化来自19种不同大鼠组织的mRNA，并利用对四种酶特异的cDNA探针通过北方印迹分析。I类乙醇脱氢酶mRNA被证明是广泛发生的，可检测到在所有组织，包括大脑，但有显着的差异量。杂交显示，II类乙醇脱氢酶mRNA的发生模式是独特的，只有在肝脏，十二指肠，肾脏，胃，脾脏和睾丸的成绩单。丰富的III类醇脱氢酶（谷胱甘肽依赖性甲醛脱氢酶）mRNA的水平存在于所有分析的组织，反映了一般需要清除甲醛的生理细胞保护。山梨醇脱氢酶的mRNA检测在所有组织中，除了小肠，在该组织中的被动扩散与山梨醇的再吸收。此外，在肝脏中，II类醇脱氢酶的性别特异性表达的证据被获得。
The tissue distribution of mRNA of alcohol dehydrogenases of classes I, II and III, and sorbitol dehydrogenase, was studied. mRNA from 19 different rat tissues was purified and analyzed by Northern blots, utilizing cDNA probes specific for the four dehydrogenases. Class-I alcohol-dehydrogenase mRNA was shown to be of widespread occurrence, detectable in all tissues including brain, but with pronounced differences in amounts. Hybridization revealed the pattern of occurrence of class-II alcohol-dehydrogenase mRNA to be unique, with transcripts only in the liver, duodenum, kidney, stomach, spleen and testis. Abundant levels of class-III alcohol-dehydrogenase (glutathione-dependent formaldehyde dehydrogenase) mRNA were present in all tissues analyzed, reflecting the general need for scavenging of formaldehyde in physiological cytoprotection. Sorbitol dehydrogenase mRNA was detected in all tissues except small intestine, in agreement with sorbitol resorbtion by passive diffusion in this tissue. In addition, evidence for a sex-specific expression, in the liver, of class-II alcohol dehydrogenase was obtained.