GDNF reduces apoptosis in dopaminergic neurons in vitro
GDNF reduces apoptosis in dopaminergic neurons in vitro
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DOI：
10.1097/00001756-199512000-00035
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发表时间：
1995-12-29
期刊：
影响因子：
1.7
通讯作者：
Freed, CR
中科院分区：
文献类型：
--
作者：
Clarkson, ED;Zawada, WM;Freed, CR
为了探讨神经胶质细胞系来源的神经营养因子（GDNF）提高细胞存活的机制，我们测量了与GDNF孵育的多巴胺神经元的凋亡率。以胚胎第15天的大鼠中脑为培养基，加入5%人胎盘血清。GDNF使多巴胺神经元的凋亡率从5%降低到2%。相比之下，GDNF对胚胎中脑和新生儿皮质星形细胞的凋亡没有影响。与胚胎纹状体以及生长因子的组合共同培养也测试了对多巴胺神经元存活的影响。与纹状体细胞共培养时，无论是否添加生长因子，神经元存活率最高。我们得出结论，GDNF抑制多巴胺神经元凋亡细胞死亡。
To explore the mechanism by which glial cell line-derived neurotrophic factor (GDNF) improves cell survival, we measured the apoptotic rate of dopamine neurons incubated with GDNF. Cultures were prepared from embryonic day 15 rat mesencephalon in medium with 5% human placental serum. GDNF reduced the rate of apoptosis in dopamine neurons from 5% to 2%. By contrast, GDNF had no effect on apoptosis in astrocytes from embryonic mesencephalon or from neonatal cortex. Co-cultures with embryonic striatum as well as with combinations of growth factors were also tested for effects on dopamine neuron survival. Neuronal survival was maximal during co-culture with striatal cells with or without added growth factors. We conclude that GDNF inhibits apoptotic cell death in dopamine neurons.