Determination of deoxyribonucleoside triphosphate concentrations in yeast cells by strong anion-exchange high-performance liquid chromatography coupled with ultraviolet detection.
Determination of deoxyribonucleoside triphosphate concentrations in yeast cells by strong anion-exchange high-performance liquid chromatography coupled with ultraviolet detection.
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DOI：
10.1007/978-1-4939-2596-4_8
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发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Chabes, Andrei
中科院分区：
文献类型：
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作者：
Jia, Shaodong;Marjavaara, Lisette;Chabes, Andrei
DNA聚合酶测定通常用于检测生物样品中的脱氧核糖核苷三磷酸（dNTP）。为了更好的特异性和准确性，高效液相色谱（HPLC）方法已被开发用于分析复杂样品中的四种dNTPs。在这里，我们描述了一个简单的方法，使用等度强阴离子交换（SAX）色谱分离结合紫外检测（UV）的芽殖酵母酿酒酵母中的四个dNTPs的分析。这种方法可以应用于其他种类的酵母或细菌。
DNA polymerase assays are commonly used for the detection of deoxyribonucleoside triphosphates (dNTPs) in biological samples. For better specificity and accuracy, high-performance liquid chromatography (HPLC) methods have been developed for the analysis of the four dNTPs in complex samples. Here we describe a simple method using isocratic strong anion-exchange (SAX) chromatographic separation coupled with ultraviolet detection (UV) for the analysis of the four dNTPs in budding yeast Saccharomyces cerevisiae. This method can be applied to other species of yeast or bacteria.