Spermidine inhibits transient and stable ribosome subunit dissociation
Spermidine inhibits transient and stable ribosome subunit dissociation
复制标题
DOI：
10.1016/j.febslet.2006.01.033
复制
发表时间：
2006-02-01
期刊：
影响因子：
3.5
通讯作者：
Ueda, T
中科院分区：
文献类型：
--
作者：
Umekage, S;Ueda, T
最近的光散射实验和蔗糖密度梯度离心分析表明，70s核糖体经历了RRF和EF-G触发的瞬时亚基解离，然后是IF3诱导的稳定解离。然而，实验条件不包括细胞内普遍存在的多胺亚精胺，这是有效翻译所必需的。我们发现，当亚精胺存在时，这种瞬时解离被抑制。此外，发表的实验使用的核糖体浓度远远低于生理浓度。我们发现，当实验条件中包括亚精胺和较高的核糖体浓度时，只观察到非常有限的稳定亚基解离。这些结果表明，在这里应用的生理条件下，既不会发生短暂的解离，也不会发生稳定的解离。(C)2006年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Recent light-scattering experiments and sucrose density gradient centrifugational analyses suggested that the 70S ribosome undergoes RRF- and EF-G-triggered transient subunit dissociation that is followed by IF3-induced stable dissociation. However, the experimental conditions did not include the ubiquitous cellular polyamine spermidine, which is required for efficient translation. We found that when spermidine was present, the transient dissociation was inhibited. Moreover, the published experiments used ribosome concentrations that were far lower than the physiological concentration. We found that when spermidine and higher ribosome concentrations were included in the experimental conditions, only very limited stable subunit dissociation was observed. These results suggest that neither transient nor stable dissociation occurs under physiological conditions applied here. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.