X-ray crystal structure of the human galectin-3 carbohydrate recognition domain at 2.1-A resolution.
X-ray crystal structure of the human galectin-3 carbohydrate recognition domain at 2.1-A resolution.
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DOI：
10.2210/pdb1a3k/pdb
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
J. Seetharaman;A. Kanigsberg;R. Slaaby;H. Leffler;S. Barondes;J. Rini
中科院分区：
文献类型：
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作者：
J. Seetharaman;A. Kanigsberg;R. Slaaby;H. Leffler;S. Barondes;J. Rini
半乳糖凝集素是具有相似的碳水化合物识别结构域（CRD）和对小β-半乳糖苷的亲和力的凝集素家族，但其对更复杂的糖缀合物的结合特异性显示出显著差异。我们在这里报告的X-射线晶体结构的人半乳糖凝集素-3 CRD，在复杂的乳糖和N-乙酰乳糖胺，在2.1-A的分辨率。该结构代表了从半乳糖凝集素确定的CRD的第一个例子，其不显示典型的2倍对称二聚体组织。与半乳糖凝集素-1和-2的已发表结构的比较提供了半乳糖凝集素-3所示的碳水化合物结合特异性差异的解释，以及它不能通过类似的CRD-CRD相互作用形成二聚体的事实。
Galectins are a family of lectins which share similar carbohydrate recognition domains (CRDs) and affinity for small beta-galactosides, but which show significant differences in binding specificity for more complex glycoconjugates. We report here the x-ray crystal structure of the human galectin-3 CRD, in complex with lactose and N-acetyllactosamine, at 2.1-A resolution. This structure represents the first example of a CRD determined from a galectin which does not show the canonical 2-fold symmetric dimer organization. Comparison with the published structures of galectins-1 and -2 provides an explanation for the differences in carbohydrate-binding specificity shown by galectin-3, and for the fact that it fails to form dimers by analogous CRD-CRD interactions.