ACTIVATED MACROPHAGES IN HUMAN PERIODONTITIS
ACTIVATED MACROPHAGES IN HUMAN PERIODONTITIS
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DOI：
10.1902/jop.1981.52.6.328
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发表时间：
1981-01-01
影响因子：
4.3
通讯作者：
GARGIULO, AW
中科院分区：
文献类型：
--
作者：
CHARON, J;TOTO, PD;GARGIULO, AW
本研究选取年龄30-88岁的晚期牙周病患者15例，男8例，女7例。取正常及炎症牙龈组织活检及血液标本，检测单核细胞及巨噬细胞非特异性酯酶及酸性磷酸酶活性。慢性牙周炎患者的牙龈上皮、固有层、血管周围组织和血管中均存在活化的巨噬细胞，对酸性磷酸酶和非特异性酯酶反应强烈。单核细胞的血液涂片显示染色强度的变异性，表明它们的激活发生在血管中，在慢性牙周炎中它们边缘并迁移到血管周围组织。然后它们以巨噬细胞的形式出现，通过结缔组织迁移，穿透基底膜并继续穿过上皮。非特异性酯酶染色通过单个点状颗粒识别T细胞，通过细胞质中的多个颗粒识别浆细胞。含有多个细胞质非特异性酯酶阳性颗粒的淋巴细胞通常仅在血管周围结缔组织中发现，可能代表b细胞向浆细胞分化。浆细胞的优势，T细胞和活化的巨噬细胞的存在表明，体液和细胞介导的反应在人类慢性牙周炎中起作用。
Fifteen patients, 8 males and 7 females, ranging from 30-88 yr of age with advanced periodontal disease were selected for this study. Biopsies and blood samples were taken of normal and inflamed gingival tissues and processed for detection of nonspecific esterase and acid phosphatase activity in monocytes and macrophages. Activated macrophages, as indicated by their intense reaction to acid phosphatase and nonspecific esterase, were found in the gingival epithelium, lamina propria, perivascular tissues and in the blood vessels in human chronic periodontitis. Blood smears of monocytes showed variability of stain intensity suggesting that their activation occurred in blood vessels where they marginate and emigrate into the perivascular tissues in chronic periodontitis. They then appear as macrophages that migrate through the connective tissue, penetrate the basement membrane and continue through the epithelium. The nonspecific esterase stain identified T cells, by a singular dot-like granule and plasma cells by multiple granules in the cytoplasm. Lymphocytes containing multiple cytoplasmic nonspecific esterase positive granules commonly were found only in the perivascular connective tissue and may represent B-cell differentiation to plasma cells. The plasma cell predominance, the presence of T cells and activated macrophages indicated that humoral and cell-mediated responses are operative in human chronic periodontitis.