Nano-hydroxyapatite particles induce apoptosis on MC3T3-E1 cells and tissue cells in SD rats.
Nano-hydroxyapatite particles induce apoptosis on MC3T3-E1 cells and tissue cells in SD rats.
复制标题
DOI：
10.1039/c2nr00044j
复制
发表时间：
2012-04
期刊：
影响因子：
6.7
通讯作者：
Liting Wang;Gang Zhou;Haifeng Liu;Xufeng Niu;Jingyun Han;Lisha Zheng;Yubo Fan
中科院分区：
文献类型：
--
作者：
Liting Wang;Gang Zhou;Haifeng Liu;Xufeng Niu;Jingyun Han;Lisha Zheng;Yubo Fan
纳米材料的优点日益被人们所认识的同时，其潜在的毒性也越来越引起人们的重视和关注。在本研究中，我们探讨了20-30 nm棒状羟基磷灰石（HA）纳米颗粒的体外和体内毒性机制。采用沉淀法制备了纳米颗粒，并用IR、XRD和TEM对其进行了表征。分别以0 μg mL（-1）、10 μg mL（-1）、100 μg mL（-1）、1 mg mL（-1）、10 mg mL（-1）的浓度作用于MC3T3-E1细胞，检测细胞活力（mtt法）。基于两种细胞死亡方法的特征差异，用透射电镜观察MC3T3-E1细胞系与纳米羟基磷灰石（n-HA） （10 mg mL(-1)）共培养24 h后的形态学特征。采用重要的血清生化指标和组织病理学检查，评价n-HA对SD大鼠（33.3 mg kg（-1）体重）腹腔注射n-HA对其主要脏器的潜在毒理学影响。结果表明，与n-HA共培养的MC3T3-E1细胞有生长抑制和凋亡现象。肝、肾组织病理切片及血清生化指标均可见凋亡，未见坏死。提示细胞凋亡可能是n-HA毒性的可能机制，并为纳米材料在人体骨修复中的生物相容性提供了更好的理解。
While the advantages of nanomaterials are being increasingly recognized, their potential toxicity is drawing more and more attention and concern. In this study, we explore the toxicity mechanism of 20-30 nm rod-shaped hydroxyapatite (HA) nanoparticles in vitro and in vivo. The nanoparticles were prepared by precipitation and characterized by IR, XRD and TEM. Concentrations of 0 μg mL(-1), 10 μg mL(-1), 100 μg mL(-1), 1 mg mL(-1), and 10 mg mL(-1) were applied to the MC3T3-E1 cells for viability (MTT-test). Based on the characteristic differences of the two methods of cell death, the morphological features of the MC3T3-E1 cell line co-cultured with nano-hydroxyapatite (n-HA) (10 mg mL(-1)) for 24 h were also observed by TEM. Furthermore, important serum biochemical markers and histopathological examinations were used to evaluate the potential toxicological effect of n-HA on the major organs of SD rats injected intraperitoneally with n-HA (33.3 mg kg(-1) body weight). In the results, we found cell growth inhibition and apoptosis in MC3T3-E1 cells co-cultured with n-HA. Moreover, apoptosis but not necrosis was illustrated in liver and renal tissue by using histopathology slices and serum biochemical markers. It suggests that apoptosis may be the possible mechanism of n-HA toxicity and provides a better understanding of the biocompatibility of nanomaterials applied in human bone repair.