Increased EMMPRIN (CD 147) expression during oral carcinogenesis
Increased EMMPRIN (CD 147) expression during oral carcinogenesis
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DOI：
10.1016/j.yexmp.2005.09.011
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发表时间：
2006-04-01
影响因子：
3.6
通讯作者：
Zacharias, W
中科院分区：
文献类型：
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作者：
Vigneswaran, N;Beckers, S;Zacharias, W
口腔癌前病变（OPM）细胞和正常口腔上皮（NOR）细胞的基因表达谱显示，EMMPRIN的表达显着上调，在OPM细胞相比，NOR细胞。我们使用了一个口腔鳞状细胞癌（OSCC）的进展模型组成的细胞系，器官型培养物和组织标本，EMMPRIN表达模式的特点，通过微阵列分析，qRT-PCR，Western印迹和免疫组化。与NOR相比，在OPM以及原发性和转移性OSCC细胞中EMMPRIN水平升高。EMMPRIN在OPM和OSCC细胞提取物中被检测为高糖基化和低糖基化形式，并且被OSCC细胞而不是OPM细胞释放到培养基中。EMMPRIN在正常和OPM粘膜的器官型培养模型中的表达反映了体内相应组织中的表达模式。EMMPRIN表达仅限于正常、良性角化过度和炎症（扁平苔藓）口腔粘膜的基底细胞。EMMPRIN表达增加，不典型增生的白斑扩展到更浅的层，其表达水平与不典型增生的程度显着相关。原发性和转移性口腔鳞癌细胞表面EMMPRIN表达较强。这些结果表明，EMMPRIN过度表达发生在口腔癌的早期阶段，并在口腔鳞癌的肿瘤发生中发挥了重要作用。(c)2005年爱思唯尔公司All rights reserved.
Gene expression profiling of oral premalignant (OPM) cells and normal oral epithelial (NOR) cells showed that EMMPRIN expression was markedly upregulated in OPM cells compared to NOR cells. We used an oral squamous cell carcinoma (OSCC) progression model composed of cell lines, organotypic cultures and tissue specimens to characterize EMMPRIN expression patterns by microarray analysis, qRT-PCR, Western blotting and immnuohistochemistry. EMMPRIN levels are elevated in OPM and primary and metastatic OSCC cells as compared to NOR. EMMPRIN was detected as high and low glycosylated forms in the OPM and OSCC cellular extracts and was released in the media by OSCC cells but not by OPM cells. EMMPRIN expression in an organotypic culture model of normal and OPM mucosae mirrored the expression patterns in the respective tissues in vivo. EMMPRIN expression was limited to basal cells of normal, benign hyperkeratotic and inflammatory (lichen planus) oral mucosa. EMMPRIN expression is increased in,dysplastic leukoplakias spreading to more superficial layers, and its expression levels correlated significantly with the degree of dysplasia. Primary and metastatic OSCC showed strong cell surface expression of EMMPRIN. These results suggest that EMMPRIN overexpression occurs at a very early stage of oral carcinogenesis and plays a contributing role in OSCC tumorigenesis. (c) 2005 Elsevier Inc. All rights reserved.