Preparation and evaluation of NGF-microsphere conduits for regeneration of defective nerves
Preparation and evaluation of NGF-microsphere conduits for regeneration of defective nerves
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DOI：
10.1179/1743132812y.0000000037
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发表时间：
2012-06-01
影响因子：
1.9
通讯作者：
Yu, Hailong
中科院分区：
文献类型：
--
作者：
Sun, Huayan;Xu, Fenghua;Yu, Hailong
目的：目的：制备神经生长因子（NGF）微球导管，并评价其对缺损神经再生的促进作用。通过酶联免疫吸附测定法测定NGF的总量，并通过PC 12细胞系方法定量生物活性NGF;监测释放期间存在的NGF生物活性。将聚乳酸制成的导管加入到NGF微球中以桥接和修复神经损伤。结果：神经生长因子微球呈球形，表面光滑致密，微球的生物学活性较好。微球的产率、包封率和载药量分别为58.3%、29.72%和0.003%。对释放期间剩余的NGF生物活性的监测表明，在第28天保持了约40%的原始生物活性，在第90天剩余10%。聚乳酸导管抗原性低，多孔（促进氧气扩散），能够防止长期压迫。15天后，神经再生长度与对照组比较差异有统计学意义（P
Objectives: To fabricate nerve growth factor (NGF) microsphere conduits and evaluate their promotional effects on regeneration of defective nerves.Methods: NGF microspheres containing ovalbumin were prepared using a double-emulsion method. The total amount of NGF was determined by enzyme-linked immunosorbent assay, and bioactive NGF was quantified by the PC12 cell line method; the NGF bioactivity present during the release period was monitored. Conduits made of polylacticacid were added to the NGF microspheres to bridge and repair the nerve injury. The sciatic nerve of Wistar rats was used to evaluate nerve-regeneration efficacy.Results: NGF microspheres were spherical, with smooth and compact surfaces. The fabrication yield, encapsulation efficiency, and drug loading of the microspheres were 58.3, 29.72, and 0.003%, respectively. The monitoring of NGF bioactivity remaining during the release period showed that similar to 40% of the original bioactivity was kept on the twenty-eighth day and 10% left on the ninetieth day. Polylacticacid conduits are lowly antigenic, porous (facilitates oxygen diffusion), and able to prevent long-term compression. After 15 days, the length of nerve regenerated from NGF-microsphere conduits was statistically different from that of the control groups (P