Effect of environmental temperature on the ability of Culex pipiens (Diptera: Culicidae) to transmit West Nile virus
Effect of environmental temperature on the ability of Culex pipiens (Diptera: Culicidae) to transmit West Nile virus
复制标题
DOI：
10.1603/0022-2585-39.1.221
复制
发表时间：
2002-01-01
影响因子：
2.1
通讯作者：
Turell, MJ
中科院分区：
文献类型：
--
作者：
Dohm, DJ;O'Guinn, ML;Turell, MJ
环境温度会影响蚊子传播虫媒病毒的能力。然而，各种研究的结果表明，这些影响在病毒或蚊子物种之间并不一致，并且没有关于环境温度对北美蚊子物种传播西尼罗河病毒（WN）能力的影响的信息。本文研究了18、20、26、30 ℃四种不同温度对尖音库蚊（Culex pipiens L.）来自1999年纽约爆发期间收集的标本，以传播从这次爆发期间死亡的乌鸦中获得的WN病毒株。尽管蚊子以相同的病毒血症鸡为食，但感染率与随后的孵化温度直接相关。在30 ℃的蚊子中，几乎所有测试的蚊子都能回收病毒，早在感染性血餐后4天就能检测到播散性感染，超过90%的蚊子在感染性血餐后12天或更长时间内有播散性感染。相比之下，对于保持在18 ℃的蚊子，直到感染性血餐后25天才检测到传播性感染，甚至在28天后，
Environmental temperature can affect the ability of mosquitoes to transmit an arbovirus. However, results of various studies indicate that these effects are not consistent among viruses or mosquito species, and there is no information available on the effect of environmental temperature on the ability of North American mosquito species to transmit West Nile (WN) virus. We evaluated the effect of incubation temperature (18, 20, 26, or 30degreesC) on the ability of Culex pipiens L. derived from specimens collected during the outbreak in New York in 1999 to transmit a strain of WN virus obtained from a crow that died during this outbreak. Although mosquitoes fed on the same viremic chickens, infection rates were directly related to subsequent incubation temperatures. In mosquitoes held at 30degreesC, virus was recovered from nearly all mosquitoes tested, disseminated infections were detected as early as 4 d after the infectious blood meal, and >90% of all mosquitoes had a disseminated infection 12 or more days after the infectious blood meal. In contrast, for mosquitoes held at 18degreesC, disseminated infections were not detected until 25 d after the infectious blood meal, and even after 28 d,