Antibodies in human sera reactive against an internal structural protein of human T-cell lymphoma virus
Antibodies in human sera reactive against an internal structural protein of human T-cell lymphoma virus
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人血清中的抗体对人 T 细胞淋巴瘤病毒的内部结构蛋白有反应
作者：
V. Kalyanaraman;M. Sarngadharan;P. Bunn;J. Minna;Robert C. Gallo
虽然逆转录病毒(RNA肿瘤病毒)已经与自然发生的动物白血病和淋巴瘤有关，但它们是否与这些恶性疾病的人类版本有关尚不清楚，特别是因为很难分离出真正归因于人类起源的病毒2。然而，我们实验室从一名患者(C.R.)的T细胞(新鲜和培养中)中分离到一种新的C型逆转录病毒(称为HTLVCR)。皮肤T细胞淋巴瘤(真菌样霉菌病)4和从另一位患者(M.B.)的外周血T细胞中提取的非常相似的病毒(HTLVMB)。皮肤T细胞白血病(S综合征，见相关报告5)。HTLVCR的核酸序列6、逆转录酶7和主要内部结构蛋白(P24)8与任何已知的逆转录病毒都没有明显的相关性；HTLVMB的核酸序列和p24蛋白已经在新鲜和培养的细胞中被识别。我们现在描述在T细胞恶性肿瘤患者(和正常人)中发生的针对HTLVCR蛋白的抗体的有限调查的结果。我们发现，抗p24的抗体存在于人的血清中(包括患者C.R.和他的妻子的血清)，并且这些抗体是针对HTLVCR蛋白的，而不是针对细胞特异性的决定因素--换句话说，这种免疫学反应不是人血清9-11中报道的针对动物病毒糖蛋白的反应，因为缺乏病毒特异性，针对糖蛋白12，13的碳水化合物残基。因此，针对HTLV的抗体是人类对逆转录病毒的特异性免疫反应的第一个证据。
Although retroviruses (RNA tumour viruses) have been implicated in the causation of naturally occurring leukaemias and lymphomas of animals1, it is not yet clear whether they are involved in the human versions of these malignant diseases, particularly because of the difficulty in isolating viruses truly ascribable to human origin2,3. However, a novel type C retrovirus (called HTLVCR) has been isolated in our laboratory from T cells (fresh and in culture) from a lymph node biopsy of a patient (C.R.) with cutaneous T-cell lymphoma (mycosis fungoides)4 and a very similar virus (HTLVMB) from the peripheral blood T cells of another patient (M.B.) with cutaneous T-cell leukaemia (Sézary syndrome, see accompanying report5). The nucleic acid sequence6, the reverse transcriptase7 and the major internal structural protein (p24)8 of HTLVCR are not significantly related to any of the known retroviruses; nucleic acid sequences and p24 protein of HTLVMB have been recognized in fresh and cultured cells5. We now describe the results of a limited survey of the occurrence, in patients with T-cell malignancies (and among normal people), of antibodies against HTLVCR proteins. We find that antibodies against p24 are present in human sera (including those of patient C.R. and his wife), and that these are specifically directed at HTLVCR proteins and not at cell-specific determinants—in other words, the immunological reactions are not those reported in human sera9–11 against animal virus glycoproteins which, lacking virus specificity, are directed against the carbohydrate residues of the glycoprotein12,13. The antibodies against HTLV are thus the first evidence for a specific immune response in humans against a retrovirus.