Gadolinium(III)-gold nanorods for MRI and photoacoustic imaging dual-modality detection of macrophages in atherosclerotic inflammation
Gadolinium(III)-gold nanorods for MRI and photoacoustic imaging dual-modality detection of macrophages in atherosclerotic inflammation
复制标题
钆（III）-金纳米棒用于动脉粥样硬化炎症中巨噬细胞的 MRI 和光声成像双模态检测。
DOI：
10.2217/nnm.12.168
复制
发表时间：
2013-10-01
期刊：
影响因子：
5.5
通讯作者：
Xing, Da
中科院分区：
文献类型：
--
作者：
Qin, Huan;Zhou, Ting;Xing, Da
目的
高危动脉粥样硬化斑块的特征之一是巨噬细胞的优势。钆（III）-金纳米棒（Gd（III）-GNRs）已被开发为用于MRI和光声成像（派）的双模态探针，以追踪巨噬细胞以确定炎症程度。
材料和方法
Gd（III）-GNRs用于活小鼠和离体模拟的富含巨噬细胞的斑块中巨噬细胞的MRI和派双模态检测。
结果
Gd（III）-GNRs在体外被巨噬细胞内吞。用派和MRI检测Gd（III）-GNRs标记的巨噬细胞。使用Gd（III）-GNRs，可以建立多尺度互补成像协议：MRI可以筛选以识别探针吞噬的巨噬细胞的位置，并且血管内派提供随后的精确形态以量化巨噬细胞在动脉壁中的浸润面积和浸润深度。
结论
这种新的双模态纳米颗粒方法有望定量检测动脉粥样硬化斑块中的巨噬细胞。
AIM
One of the features of high-risk atherosclerotic plaques is the preponderance of macrophages. Gadolinium(III)-gold nanorods (Gd(III)-GNRs) have been developed as a dual-modality probe for MRI and photoacoustic imaging (PAI) to trace macrophages for determining the degree of inflammation.
MATERIALS & METHODS
Gd(III)-GNRs were utilized for MRI and PAI dual-modality detection of macrophages in living mice and ex vivo simulated macrophage-rich plaque.
RESULTS
Gd(III)-GNRs were shown to be endocytosed by macrophages in vitro. Macrophages labeled with Gd(III)-GNRs were detected by both PAI and MRI. With Gd(III)-GNRs, it is possible to institute a multiscale complementary imaging protocol: MRI can screen to identify the location of the probe-phagocytosed macrophages, and intravascular PAI provides a subsequent precise morphology to quantify the infiltration area and invasion depth of macrophages in the arterial wall.
CONCLUSION
This new dual-modality nanoparticle approach has promise for enabling quantitative detection of macrophages in atherosclerotic plaques.