Genome-wide DNA methylation profiles of porcine ovaries in estrus and proestrus
Genome-wide DNA methylation profiles of porcine ovaries in estrus and proestrus
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发情期和发情前期猪卵巢的全基因组 DNA 甲基化谱
DOI：
10.1152/physiolgenomics.00052.2017
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Zhao ayong
中科院分区：
文献类型：
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作者：
Zhao xiaolong;Yang songbai;Yan feifei;Heke;Zhao ayong
DNA甲基化是一种重要的表观遗传修饰，参与了动情周期和生殖调控。在这里，我们调查了全基因组范围内的DNA甲基化在发情前期和发情期猪卵巢的甲基化DNA免疫沉淀测序。结果表明，DNA甲基化主要集中在基因间区和内含子区。基因编码区甲基化水平高于5′-和3′-侧翼区。在发情期与发情前期的卵巢基因组中，有4，813个CpG岛差异甲基化区域（DMR）。此外，在发情期和发情前期的猪中鉴定了3，651个差异甲基化基因（DMG）。DMG在与生殖和激素调节相关的生物过程和途径中显着富集。我们确定了90 DMG与调节猪的繁殖。我们的研究结果可以作为资源的DNA甲基化研究集中在猪卵巢和进一步了解表观遗传调控的哺乳动物繁殖。
DNA methylation is an important epigenetic modification involved in the estrous cycle and the regulation of reproduction. Here, we investigated the genome-wide profiles of DNA methylation in porcine ovaries in proestrus and estrus using methylated DNA immunoprecipitation sequencing. The results showed that DNA methylation was enriched in intergenic and intron regions. The methylation levels of coding regions were higher than those of the 5′- and 3′-flanking regions of genes. There were 4,813 differentially methylated regions (DMRs) of CpG islands in the estrus vs. proestrus ovarian genomes. Additionally, 3,651 differentially methylated genes (DMGs) were identified in pigs in estrus and proestrus. The DMGs were significantly enriched in biological processes and pathways related to reproduction and hormone regulation. We identified 90 DMGs associated with regulating reproduction in pigs. Our findings can serve as resources for DNA methylome research focused on porcine ovaries and further our understanding of epigenetically regulated reproduction in mammals.