Maternal and postnatal vitamin D ingestion influences rat aortic structure, function and elastin content
Maternal and postnatal vitamin D ingestion influences rat aortic structure, function and elastin content
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DOI：
10.1016/s0008-6363(02)00444-3
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发表时间：
2002-08-01
影响因子：
10.8
通讯作者：
Powell, J
中科院分区：
文献类型：
--
作者：
Norman, P;Moss, I;Powell, J
胎儿营养的轻微损害似乎预示着成年人的高血压和动脉粥样硬化。假设主动脉弹性发生受损是成人高血压和主动脉瘤的起始步骤。维生素D已被证明可以抑制培养的平滑肌细胞合成弹性蛋白。在这里，我们已经调查，在大鼠中，假设增加暴露于维生素D在妊娠期和出生后期间改变主动脉弹性蛋白含量和主动脉功能。研究方法：在妊娠期和哺乳期，将9对繁殖的Sprague-Dawley大鼠分配至含3000（对照组）、6000（低剂量）或12，000（高剂量）IU/kg维生素D的三种饲料中的一种。雄性幼仔继续接受相同的饮食，直至6周龄。通过使用毛细管区带电泳测量锁链素+异锁链素含量来评估主动脉弹性蛋白含量。主动脉横切面用于弹性层定量和形态计量学分析。在器官浴制备中评估主动脉环的收缩性。结果如下：对照组（n=20）、低剂量组（n = 23）和高剂量组（n=15）6周龄雄性幼仔腹主动脉的锁链素+异锁链素含量分别为14.1、10.0和10.1 nmol/mg干重（P=0.007）。对照组、低剂量组和高剂量组的胸主动脉远端弹性膜数中位数分别为8.25、7.13和6.88（P
Subtle impairment of fetal nutrition appears to predict hypertension and atherosclerosis in adults. It has been hypothesised that impaired aortic elastogenesis is the initiating step in adult hypertension and aortic aneurysms. Vitamin D has been shown to inhibit elastin synthesis by cultured smooth muscle cells. Here we have investigated, in rats, the hypothesis that increased exposure to vitamin D during gestation and in the postnatal period alters aortic elastin content and aortic function. Methods: Nine breeding pairs of Sprague-Dawley rats were allocated to one of three diets containing 3000 (control group), 6000 (low dose) or 12,000 (high dose) IU/kg vitamin D during pregnancy and lactation. Male pups were continued on the same diet until 6 weeks of age. Aortic elastin content was assessed by measuring desmosine+isodesmosine content using capillary zone electrophoresis. Transverse aortic sections were used for quantification of elastic lamellae and morphometric analysis. The contractility of aortic rings was assessed in an organ bath preparation. Results: The desmosine+isodesmosine content of the abdominal aorta of 6-week-old male pups, was 14.1, 10.0 and 10.1 nmol/mg dry weight in the control (n=20), low- (n=23) and high-dose (n=15) groups, respectively (P=0.007). The median number of elastic lamellae of the distal thoracic aorta was 8.25, 7.13 and 6.88 in the control, low-dose and high-dose groups, respectively (P