STORAGE STABILITY OF DIFFERENT ANTIBODY SPECIES AGAINST ARBOVIURS AND RICKETTSIAL ANTIGENS IN BLOOD DRIED ON FILTER PAPER DISCS
STORAGE STABILITY OF DIFFERENT ANTIBODY SPECIES AGAINST ARBOVIURS AND RICKETTSIAL ANTIGENS IN BLOOD DRIED ON FILTER PAPER DISCS
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DOI：
10.1093/oxfordjournals.aje.a120947
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发表时间：
1969-01-01
影响因子：
5
通讯作者：
WISSEMAN, CL
中科院分区：
文献类型：
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作者：
COHEN, AB;HATGI, JN;WISSEMAN, CL
将含有黄热病病毒血凝抑制（HI）抗体（主要为伊加、IgM或IgG免疫球蛋白类）、分布在IgG和伊加或IgM免疫球蛋白之间的流行性斑疹伤寒凝集（MA）抗体以及IgG免疫球蛋白类中的斑疹伤寒补体结合（CF）抗体的柠檬酸盐人血在滤纸片上干燥。在密封在防潮袋中之后，将盘组储存在-20、+4、+26和+37 ℃下。在干燥后，每隔一段时间从圆盘制备的洗脱液测试特异性抗体活性的恢复。IgM免疫球蛋白的抗体活性在储存后迅速下降，与温度无关。因此，即使在-20 ℃下储存1个月的洗脱液中也检测不到属于IgM免疫球蛋白类的黄热病HI抗体。相比之下，存在于IgG免疫球蛋白类别中的抗体活性在4个月内在滤纸片上似乎相对稳定，尤其是在较低温度下储存时。伊加抗体的稳定性需要进一步研究。从采用滤纸片技术的研究中获得的血清学数据的解释必须考虑到不同类别的免疫球蛋白在储存稳定性方面的差异。
Citrated human bloods containing yellow fever virus hemagglutination-inhi biting (HI) antibodies predominantly in the IgA, IgM or IgG immunoglobulin classes, epidemic typhus agglutinating (MA) antibodies distributed between IgG and either IgA or IgM immunoglobulins and typhus complement fixing (CF) antibodies in the IgG immunoglobulin class were dried on filter paper discs. After sealing in moisture-proof bags, groups of discs were stored at –20, +4, +26 and +37 C. EJuates prepared from the discs at intervals after drying were tested for the recovery of specific antibody activity. Antibody activity attributable to IgM immunoglobulin declined rapidly upon storage, regardless of temperature. Thus, yellow fever HI antibodies belonging to the IgM immunoglobulin class were not detectable in eluates which had been stored for 1 month, even at —20 C. In contrast, antibody activity residing in the IgG immunoglobulin class appeared to be relatively stable on filter paper discs over a 4 month period, especially when stored at lower temperatures. Stability of IgA antibodies requires further study. Interpretation of serologic data obtained from studies employing the filter paper disc technique must take into account differences in storage stability of the various classes of immunogbbulins.