Bazooka regulates microtubule organization and spatial restriction of germ plasm assembly in the Drosophila oocyte
Bazooka regulates microtubule organization and spatial restriction of germ plasm assembly in the Drosophila oocyte
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DOI：
10.1016/j.ydbio.2010.02.006
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发表时间：
2010-04-15
影响因子：
2.7
通讯作者：
Gavis, Elizabeth R.
中科院分区：
文献类型：
--
作者：
Becalska, Agata N.;Gavis, Elizabeth R.
在胚胎发育过程中，将种质定位到果蝇卵母细胞的后部是前后构型和生殖细胞发育所必需的。虽然控制个体种质成分定位的机制已经被阐明，但种质组装被限制在后极处的过程却知之甚少。在这项研究中，我们发现了一个新的火箭筒(Baz)等位基因，即PAR-3的果蝇同源基因，这使得对Baz在整个卵子发生过程中的功能进行了分析。我们证明了BAZ对于种质的空间限制和轴模式是必需的，并且我们揭示了BAZ在调节卵母细胞微管细胞骨架的组织方面的多重要求。我们的结果表明，PAR蛋白建立的不同的皮质结构域通过对微管组织的不同影响而使卵母细胞极化。我们进一步表明，即使在距离卵母细胞皮质一段距离的地方，微管+末端的丰富也足以驱动生殖质组装，这表明微管组织的控制不仅对于生殖质成分定位到卵母细胞的后部至关重要，而且对于将生殖质组装限制在后极也是至关重要的。(C)2010 Elsevier Inc.保留所有权利。
Localization of the germ plasm to the posterior of the Drosophila oocyte is required for anteroposterior patterning and germ cell development during embryogenesis. While mechanisms governing the localization of individual germ plasm components have been elucidated, the process by which germ plasm assembly is restricted to the posterior pole is poorly understood. In this study, we identify a novel allele of bazooka (baz), the Drosophila homolog of Par-3, which has allowed the analysis of baz function throughout oogenesis. We demonstrate that baz is required for spatial restriction of the germ plasm and axis patterning, and we uncover multiple requirements for baz in regulating the organization of the oocyte microtubule cytoskeleton. Our results suggest that distinct cortical domains established by Par proteins polarize the oocyte through differential effects on microtubule organization. We further show that microtubule plus-end enrichment is sufficient to drive germ plasm assembly even at a distance from the oocyte cortex, suggesting that control of microtubule organization is critical not only for the localization of germ plasm components to the posterior of the oocyte but also for the restriction of germ plasm assembly to the posterior pole. (C) 2010 Elsevier Inc. All rights reserved.